HSP60 cDNA ORF Clone, Human, N-GFPSpark® tag

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HSP60 cDNA ORF Clone, Human, N-GFPSpark® tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
1722 bp
序列特征
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 798 T/C, 1002 A/G not causing the amino acid variation.
产品特征
Full length Clone DNA of Human heat shock 60kDa protein 1 (chaperonin) with N terminal GFPSpark tag.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI (6kb + 2.45kb)
标签序列
GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

HSP60 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

HSP60 cDNA ORF Clone, Human, N-GFPSpark® tag: 验证图片

HSP60 cDNA ORF Clone, Human, N-GFPSpark® tag: 别称

CPN60 cDNA ORF Clone, Human; GROEL cDNA ORF Clone, Human; HLD4 cDNA ORF Clone, Human; HSP-60 cDNA ORF Clone, Human; HSP60 cDNA ORF Clone, Human; HSP65 cDNA ORF Clone, Human; HuCHA60 cDNA ORF Clone, Human; SPG13 cDNA ORF Clone, Human

HSP60 背景信息

HSPD1, also known as HSP6, is a member of the chaperonin family. HSPD1 may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. It may also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. HSPD1 gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13). Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4); also called mitochondrial HSP6 chaperonopathy or MitCHAP-6 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. HSPD1 is cinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
全称
heat shock 60kDa protein 1 (chaperonin)
参考文献
  • Hansen J J, et al. (2002) Hereditary spastic paraplegia SPG13 is associated with a mutation in the gene encoding the mitochondrial chaperonin Hsp60. Am J Hum Genet. 70: 1328-32.
  • Magen D, et al. (2008) Mitochondrial Hsp60 chaperonopathy causes an autosomal-recessive neurodegenerative disorder linked to brain hypomyelination and leukodystrophy. Am J Hum Genet. 83: 30-42.
  • Venner TJ, et al. (1990) Nucleotide sequences and novel structural features of human and Chinese hamster hsp60 (chaperonin) gene families. DNA Cell Biol. 9 (8): 545-52.
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