输入关键词搜索之后,可以在左侧选择分子或者产品类型以精简过滤器,快速查找产品。如果有任何问题,请 联系我们!
Text Size:AAA

人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), C-Flag 标签

产品数据评论相关产品实验方法
Human IDO1 cDNA Clone产品信息
NCBI注册码:NM_002164.4
参考序列ORF长度:1212bp
cDNA基因描述:Full length Clone DNA of Homo sapiens indoleamine 2,3-dioxygenase 1 with C terminal Flag tag.
分子别称:IDO, INDO, IDO1
分子种属:Human
载体:pCMV3-C-FLAG
质粒:pCMV3-IDO1-Flag
限制性酶切位点:KpnI + XbaI (6kb + 1.25kb)
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human IDO1 基因质粒图
Human IDO1 natural ORF mammalian expression plasmid, C-Flag tag
Human IDO1 基因表达验证图
Human IDO1 ORF mammalian expression plasmid, C-Flag tag
[点击查看大图]
Caption:
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), C-Flag 标签 on other vectors
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG11650-ACG1950
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG11650-ACR1950
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG11650-ANG1950
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG11650-ANR1950
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG11650-CF1770
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), C-His 标签HG11650-CH1770
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG11650-CM1770
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG11650-CY1770
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA(克隆载体)HG11650-M760
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG11650-NF1770
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), N-His 标签HG11650-NH1770
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG11650-NM1770
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG11650-NY1770
人 IDO1/Indoleamine 2,3‑dioxygenase 基因ORF全长cDNA克隆(表达载体)HG11650-UT1770
 点击了解更多载体信息
Product nameProduct name
研究背景

Indoleamine 2,3-dioxygenase-1, also known as Indoleamine-pyrrole 2,3-dioxygenase, IDO1 and IDO, is a member of the indoleamine 2,3-dioxygenase family. IDO1 / IDO and tryptophan 2,3-dioxygenase (TDO) are tryptophan-degrading enzymes that catalyze the first step in tryptophan catabolism via the kynurenine pathway. TDO is widely distributed in both eukaryotes and bacteria. In contrast, IDO has been found only in mammals and yeast. In 2007, a third enzyme, indoleamine 2,3-dioxygenase-2 (IDO2), was discovered. IDO2 is found not only in mammals but also in lower vertebrates. IDO1 / IDO is an immunosuppressive molecule inducible in various cells. IDO1 / IDO catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. It mediates oxidative cleavage of tryptophan, an amino acid essential for cell proliferation and survival. IDO1 / IDO inhibition is proposed to have therapeutic potential in immunodeficiency-associated abnormalities, including cancer. The IDO pathway is activated in multiple tumor types. Selective inhibition of IDO1 may represent an attractive cancer therapeutic strategy via up-regulation of cellular immunity. IDO1 / IDO is an enzyme that suppresses adaptive T-cell immunity by catabolizing tryptophan from the cellular microenvironment. Inhibition of IDO pathway might enhance the efficacy of immunotherapeutic strategies for cancer.

参考资料
  • Barnes NA. et al., 2009, J Immunol. 183 (9): 5768-77.
  • Yuasa HJ. et al., 2009, Comp Biochem Physiol B Biochem Mol Biol. 153 (2): 137-44.
  • L b S. et al., 2009, Cancer Immunol Immunother. 58 (1): 153-7.
  • Liu,X. et al., 2010, Blood.115 (17): 3520-30.
  • Sun,T. et al., 2010, Mol Cell Biochem. 342 (1-2): 29-34.
  • Kiank C. et al., 2010, PLoS One. 5 (7): e11825.
  • Size / Price
    货号: HG11650-CF
    目录价: 
    单价:      (You Save: )
    规格:
    数量:+-
    库存In Stock
    大包装查询加入购物车
    Contact Us
    产品图片
    • Human IDO1 natural ORF mammalian expression plasmid, C-Flag tag
    • Human IDO1 ORF mammalian expression plasmid, C-Flag tag
      产品数据可能随批次变化,最新数据请详见随货说明书。
      请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”