IDO cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG11650-CF
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All IDO Reagents
CRO Services
Product recommended

All IDO Reagents
IDO cDNA ORF Clone, Human, C-DDK (Flag®) tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
1212 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human indoleamine 2,3-dioxygenase 1 with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-FLAG
限制性酶切位点
KpnI + XbaI (6kb + 1.25kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
IDO cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

IDO cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images
Human IDO1/Indoleamine 2,3‑dioxygenase Gene Expression validated Image 1839
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
other Green fluorescent protein / GFP Gene Plasmid Map 5609
IDO cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
IDO cDNA ORF Clone, Human;IDO-1 cDNA ORF Clone, Human;INDO cDNA ORF Clone, Human;Indoleamine 2,3‑dioxygenase cDNA ORF Clone, Human
IDO Background Information

Indoleamine 2,3-dioxygenase-1, also known as Indoleamine-pyrrole 2,3-dioxygenase, IDO1 and IDO, is a member of the indoleamine 2,3-dioxygenase family. IDO1 / IDO and tryptophan 2,3-dioxygenase (TDO) are tryptophan-degrading enzymes that catalyze the first step in tryptophan catabolism via the kynurenine pathway. TDO is widely distributed in both eukaryotes and bacteria. In contrast, IDO has been found only in mammals and yeast. In 2007, a third enzyme, indoleamine 2,3-dioxygenase-2 (IDO2), was discovered. IDO2 is found not only in mammals but also in lower vertebrates. IDO1 / IDO is an immunosuppressive molecule inducible in various cells. IDO1 / IDO catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. It mediates oxidative cleavage of tryptophan, an amino acid essential for cell proliferation and survival. IDO1 / IDO inhibition is proposed to have therapeutic potential in immunodeficiency-associated abnormalities, including cancer. The IDO pathway is activated in multiple tumor types. Selective inhibition of IDO1 may represent an attractive cancer therapeutic strategy via up-regulation of cellular immunity. IDO1 / IDO is an enzyme that suppresses adaptive T-cell immunity by catabolizing tryptophan from the cellular microenvironment. Inhibition of IDO pathway might enhance the efficacy of immunotherapeutic strategies for cancer.

Immune Checkpoint
Immune Checkpoint Detection: ELISA Antibodies
Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
indoleamine 2,3-dioxygenase 1
References
  • Barnes NA. et al., 2009, J Immunol. 183 (9): 5768-77.
  • Yuasa HJ. et al., 2009, Comp Biochem Physiol B Biochem Mol Biol. 153 (2): 137-44.
  • L b S. et al., 2009, Cancer Immunol Immunother. 58 (1): 153-7.
  • Liu,X. et al., 2010, Blood.115 (17): 3520-30.
  • Sun,T. et al., 2010, Mol Cell Biochem. 342 (1-2): 29-34.
  • Kiank C. et al., 2010, PLoS One. 5 (7): e11825.
  • IDO cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    IDO cDNA ORF Clone, Rat, untagged RG82547-UT
    IDO cDNA ORF Clone, Rat, C-GFPSpark® tag RG82547-ACG
    IDO Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG82547-ACGLN
    IDO cDNA ORF Clone, Rat, N-GFPSpark® tag RG82547-ANG
    IDO cDNA ORF Clone, Rat, C-OFPSpark® tag RG82547-ACR
    IDO cDNA ORF Clone, Rat, N-OFPSpark® tag RG82547-ANR
    IDO cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG82547-CF
    IDO cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG82547-NF
    IDO cDNA ORF Clone, Rat, C-His tag RG82547-CH
    IDO cDNA ORF Clone, Rat, N-His tag RG82547-NH
    IDO cDNA ORF Clone, Rat, C-HA tag RG82547-CY
    IDO cDNA ORF Clone, Rat, N-HA tag RG82547-NY
    IDO cDNA ORF Clone, Rat, C-Myc tag RG82547-CM
    IDO cDNA ORF Clone, Rat, N-Myc tag RG82547-NM

    IDO cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    IDO cDNA ORF Clone, Mouse, untagged MG57213-UT
    IDO Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG57213-ACGLN
    IDO cDNA ORF Clone, Mouse, C-GFPSpark® tag MG57213-ACG
    IDO cDNA ORF Clone, Mouse, N-GFPSpark® tag MG57213-ANG
    IDO cDNA ORF Clone, Mouse, C-OFPSpark® tag MG57213-ACR
    IDO cDNA ORF Clone, Mouse, N-OFPSpark® tag MG57213-ANR
    IDO cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG57213-CF
    IDO cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG57213-NF
    IDO cDNA ORF Clone, Mouse, C-His tag MG57213-CH
    IDO cDNA ORF Clone, Mouse, N-His tag MG57213-NH
    IDO cDNA ORF Clone, Mouse, C-HA tag MG57213-CY
    IDO cDNA ORF Clone, Mouse, N-HA tag MG57213-NY
    IDO cDNA ORF Clone, Mouse, C-Myc tag MG57213-CM
    IDO cDNA ORF Clone, Mouse, N-Myc tag MG57213-NM

    IDO cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    IDO cDNA ORF Clone in Cloning Vector, Human HG11650-M
    IDO cDNA ORF Clone, Human, untagged HG11650-UT
    IDO cDNA ORF Clone, Human, C-GFPSpark® tag HG11650-ACG
    IDO Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11650-ACGLN
    IDO cDNA ORF Clone, Human, N-GFPSpark® tag HG11650-ANG
    IDO cDNA ORF Clone, Human, C-OFPSpark® tag HG11650-ACR
    IDO cDNA ORF Clone, Human, N-OFPSpark® tag HG11650-ANR
    IDO cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11650-NF
    IDO cDNA ORF Clone, Human, C-His tag HG11650-CH
    IDO cDNA ORF Clone, Human, N-His tag HG11650-NH
    IDO cDNA ORF Clone, Human, C-HA tag HG11650-CY
    IDO cDNA ORF Clone, Human, N-HA tag HG11650-NY
    IDO cDNA ORF Clone, Human, C-Myc tag HG11650-CM
    IDO cDNA ORF Clone, Human, N-Myc tag HG11650-NM
    IDO qPCR Primer Pairs, Human HP101496
    info info
    添加购物车成功! 添加购物车失败!