Human IL17RB HEK293 Overexpression Lysate

Cat: 13091-H08HL

Contact us:

215-583-7898
order@sinobiological.com
Distributors

All IL17RB Reagents

CRO Services

Product recommended


All IL17RB Reagents

Human IL17RB HEK293 Overexpression Lysate 产品信息

Product Description
This Human IL17RB overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL17RB protein (Cat: 13091-H08H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Species
Human
Sequence Information
A DNA sequence encoding the human IL17BR (NP_061195.2) (Met1-Gly289) was expressed with a C-terminal polyhistidine tag.
Molecule Mass
The recombinant human IL17BR comprises 284 amino acids and has a predicted molecular mass of 31.5 kDa. The apparent molecular mass of the protein is approximately 41-45 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Human IL17RB HEK293 Overexpression Lysate Usage Guide

Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human IL17RB HEK293 Overexpression Lysate Alternative Names

Human CRL4 Overexpression Lysate;Human EVI27 Overexpression Lysate;Human IL17BR Overexpression Lysate;Human IL17RH1 Overexpression Lysate

IL17RB Background Information

Full Name
interleukin 17 receptor B
References
  • Rickel EA, et al.. (2008) Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. J Immunol. 181(6): 4299-310.
  • Stock P, et al.. (2009) Induction of airway hyperreactivity by IL-25 is dependent on a subset of invariant NKT cells expressing IL-17RB. J Immunol. 182(8): 5116-22.
  • Wang H, et al.. (2010) Allergen challenge of peripheral blood mononuclear cells from patients with seasonal allergic rhinitis increases IL-17RB, which regulates basophil apoptosis and degranulation. Clin Exp Allergy. 40(8): 1194-202.
  • info info
    添加购物车成功! 添加购物车失败!