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人 IL-34 基因ORF全长cDNA克隆(表达载体)

  • Human IL34 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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 IL34 cDNA Clone产品信息
NCBI注册码:NM_152456.1
参考序列ORF长度:729bp
cDNA基因描述:Full length Clone DNA of Homo sapiens interleukin 34.
分子别称:IL-34, C16orf77, MGC34647
分子种属:Human
载体:pCMV3-untagged
质粒:pCMV3-IL34
限制性酶切位点:KpnI + XbaI (6.1kb + 0.73kb)
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with IL34 qPCR primers for gene expression analysis, HP100825 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Ampicillin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
 IL34 基因质粒图
Human IL34 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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研究背景

Interleukin 34 (IL-34), also known as uncharacterized protein C16 or f77 homolog, is a secreted cytokine that belongs to unknown cytokine family. IL-34 protein is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate and colon, and most abundant in the spleen. The colony-stimulating factor 1 receptor (CSF-1R) is identified as the receptor for IL-34. IL-34 increases growth or survival of immune cells known as monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.

参考资料
  • Lin H, et al. (2008) Discovery of a cytokine and its receptor by functional screening of the extracellular proteome. Science. 320(5877): 807-11.
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    货号: HG10948-UT
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