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人 IL7Ra / CD127 基因全长ORF克隆 (表达载体), C端FLAG标签

产品数据评论相关产品实验方法
Human IL7R cDNA Clone产品信息
NCBI注册码:NM_002185.2
参考序列ORF长度:1380bp
cDNA基因描述:Full length Clone DNA of Homo sapiens interleukin 7 receptor with C terminal Flag tag.
分子别称:ILRA, CD127, IL7RA, CDW127, IL-7R-alpha, IL7R
分子种属:Human
载体:pCMV3-C-FLAG
质粒:pCMV3-IL7R-Flag
限制性酶切位点:KpnI + XbaI (6kb + 1.42kb)
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human IL7R 基因质粒图
Human IL7Ra / CD127 natural ORF mammalian expression plasmid, C-Flag tag
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Product nameProduct name
重组小鼠 CD122 / IL2RB / IL2 Receptor beta 蛋白 (His 标签)重组狗 IL3RA 蛋白 (His 标签)重组狗 IL2RB / IL2 Receptor beta 蛋白 (Fc 标签)重组大鼠 IL7 / interleukin 7 蛋白重组人 IL-15 / IL15 / Interleukin 15 蛋白 (His 标签)Mouse CD123 / IL3RA Protein (ECD, Fc Tag)重组人 IL3 / IL-3 蛋白 (His 标签)重组食蟹猴 / 恒河猴 IL21R / IL-21R 蛋白 (Fc 标签)重组人 IL3 / IL-3 蛋白重组小鼠 IL-21R / Il21R 蛋白 (ECD, His Tag)重组大鼠 IL9 / IL-9 蛋白 (His 标签)Rhesus CD122 / IL-2RB Protein (Fc Tag)重组人 IL-8 / CXCL8 Protein (aa 28-99)Rhesus CD122 / IL-2RB Protein (His Tag)重组小鼠 IL7 / Interleukin 7 蛋白重组人 IL-8 / CXCL8 蛋白 (aa 23-99, Fc 标签)重组人 IL-8 / CXCL8 蛋白 (aa 28-99, Fc 标签)重组人 IL-8 / CXCL8 蛋白 (aa 23-99)重组人 IL-8 / CXCL8 蛋白 (aa 28-99)重组人 IL2Ra / CD25 蛋白 (Fc 标签)重组人 IL2Ra / CD25 蛋白 (His 标签)重组人 IL13RA2 / IL13R 蛋白 (His & Fc 标签)重组人 IL13RA2 / CD213A2 蛋白 (His 标签)重组人 IL13 / ALRH 蛋白 (Fc 标签)重组人 IL13 / ALRH 蛋白重组人 IL5Ra / CD125 蛋白 (His 标签)重组人 IL4R / CD124 蛋白 (His 标签)重组人 CD131 / CSF2RB / IL3RB / IL5RB 蛋白 (His 标签)重组人 IL3RA / CD123 蛋白 (His & Fc 标签)重组人 IL3RA / CD123 蛋白 (His 标签)重组小鼠 CD123 / IL3RA 蛋白 (ECD, His 标签)重组人 IL2RG / CD132 蛋白 (Fc 标签)重组人 IL2RG / CD132 蛋白 (His 标签)重组人 Interleukin-21 / IL-21 蛋白重组人 CD122 / IL-2RB 蛋白 (Fc 标签)重组人 CD122 / IL-2RB 蛋白重组人 IL13RA1 蛋白 (His & Fc 标签)重组人 IL13RA1 蛋白 (His 标签)重组人 IL16 / Interleukin-16 蛋白 (His 标签)重组人 IL7RA / CD127 蛋白 (His & Fc 标签)重组食蟹猴 CD127 / IL-7RA 蛋白 (His 标签)重组人 IL7RA / CD127 蛋白 (His 标签)重组食蟹猴 IL13 / ALRH 蛋白 (His 标签)重组食蟹猴 IL13 / ALRH 蛋白重组人 Interleukin-32 / IL-32 蛋白 (isoform alpha, His 标签)重组人 IL-21R / Interleukin-21 Receptor 蛋白 (His 标签)重组人 IL7 / interleukin 7 蛋白重组人 IL-9 / Interleukin-9 蛋白 (His 标签)重组人 IL4 / Interleukin-4 蛋白重组人 IL-3 / Interleukin-3 蛋白 (His 标签)重组小鼠 IL-4R / CD124 蛋白 (ECD, His 标签)重组小鼠 IL4 / Interleukin-4 蛋白重组小鼠 IL-34 蛋白 (His 标签)重组小鼠 IL13RA2 / CD213A2 蛋白 (His & Fc 标签)重组小鼠 IL13RA2 / CD213A2 蛋白 (His 标签)重组小鼠 IL2RG 蛋白 (His & Fc 标签)重组小鼠 IL2RG / CD132 蛋白 (His 标签)重组小鼠 IL-13Ra1 蛋白 (His & Fc 标签)重组小鼠 IL13RA1 蛋白 (His 标签)重组小鼠 IL7RA / CD127 蛋白 (His 标签)重组小鼠 IL21 / Interleukin 21 蛋白重组小鼠 IL13 / ALRH 蛋白重组小鼠 IL2RA / CD25 蛋白 (His 标签)重组小鼠 Interleukin-2 / IL-2 蛋白重组小鼠 IL5 蛋白 (His 标签)重组小鼠 IL4 / Interleukin-4 蛋白 (Q136D, Y139D, His 标签)重组小鼠 IL5Ra / CD125 蛋白 (His 标签)重组狗 IL-8 / CXCL8 蛋白重组狗 Interleukin-2 / IL-2 蛋白 (147 Cys/Ser)重组狗 IL21 / Interleukin 21 蛋白重组狗 IL5 蛋白 (His 标签)重组狗 IL4 / Interleukin-4 蛋白重组狗 IL13RA2 / IL13R 蛋白 (His 标签)重组人 IL5 / Interleukin 5 蛋白重组大鼠 Interleukin-2 / IL-2 蛋白重组大鼠 IL9R / Interleukin 9 receptor 蛋白 (His 标签)重组大鼠 IL7R / IL7RA 蛋白 (Fc 标签)重组大鼠 IL7R / IL7RA 蛋白 (His 标签)重组大鼠 IL13RA1 蛋白 (Fc 标签)重组大鼠 IL-21R / Interleukin-21 Receptor 蛋白 (His 标签)重组大鼠 IL2RG / CD132 蛋白 (Fc 标签)重组大鼠 IL2RG / CD132 蛋白 (His 标签)重组大鼠 IL4R / Il4ra 蛋白 (Fc 标签)重组大鼠 IL4R / Il4ra 蛋白 (His 标签)重组大鼠 IL21 / Interleukin 21 蛋白 (His 标签)重组大鼠 IL13RA2 / IL13R 蛋白 (Fc 标签)重组大鼠 IL13RA2 / IL13R 蛋白 (His 标签)重组大鼠 IL3 / interleukin 3 蛋白 (His 标签)重组大鼠 CD131 / CSF2RB / IL3RB / IL5RB 蛋白 (Fc 标签)重组人 IL2 / Interleukin-2 蛋白 (L35M, L36S, C142A)重组食蟹猴 IL-21R / Interleukin-21 Receptor 蛋白 (His 标签)Human IL-15 / IL15 / Interleukin 15 Protein重组食蟹猴 IL2RA 蛋白 (Fc 标签)重组食蟹猴 IL2RA 蛋白 (His 标签)重组食蟹猴 IL2RA 蛋白重组食蟹猴 IL-8 / CXCL8 蛋白Human IL-8 / CXCL8 Protein (His Tag)重组小鼠 IL16 / Interleukin-16 蛋白 (His 标签)重组狗 IL13RA2 / IL13R 蛋白 (Fc 标签)
研究背景

Interleukin 7 Receptor alpha (IL-7RA), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7 receptor alpha (CD127) signaling is essential for T-cell development and regulation of naive and memory T-cell homeostasis. IL-7RA is critically required for the proper development and function of lymphoid cells. Therefore, the IL-7RA is critically required for the proper development and function of lymphoid cells. Studies from both pathogenic and controlled HIV infection indicate that the containment of immune activation and preservation of CD127 expression are critical to the stability of CD4(+) T cells in infection. A better understanding of the factors regulating CD127 expression in HIV disease, particularly on T(CM) cells, might unveil new approaches exploiting the IL-7/IL-7R receptor pathway to restore T cell homeostasis and promote immune reconstitution in HIV infection. Factors relevant to HIV infection that could potentially decrease CD127 expression on human CD8(+) T cells. CD127 down-regulation may be an important contributor to HIV-associated T-cell dysfunction. In addition to IL-7, IL-7RA also associates with TSLPR to form the functional receptor for thymic stromal lymphopoietin (TSLP) which indirectly regulates T cell development by modulating dendritic cell activation. Mutations in the human IL-7RA gene cause a type of severe combined immunodeficiency in which the major deficiencies are in T cell development, whereas B and NK cells are relatively normal in number. Variation in the IL7RA gene was recently found associated with multiple sclerosis (MS). The polymorphisms in the IL7RA gene is involved in MS pathogenesis and suggest that IL7RA variation may primarily affect chronic disease courses. Soluble CD127 (sCD127) appears to play an important role in the immunopathogenesis of several chronic infections, multiple sclerosis, and various cancers.

参考资料
  • Vranjkovic A, et al. (2007) IL-7 decreases IL-7 receptor alpha (CD127) expression and induces the shedding of CD127 by human CD8+ T cells. Int Immunol. 19(12): 1329-39.
  • Kiazyk SA, et al. (2008) Loss of CD127 expression links immune activation and CD4(+) T cell loss in HIV infection. Trends Microbiol. 16(12): 567-73.
  • Akkad DA, et al. (2009) Variation in the IL7RA and IL2RA genes in German multiple sclerosis patients. J Autoimmun. 32(2): 110-5.
  • Crawley AM, et al. (2010) Soluble IL-7R alpha (sCD127) inhibits IL-7 activity and is increased in HIV infection. J Immunol. 184(9): 4679-87.
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    货号: HG10975-CF
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