|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 ING5 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG15462-ACG|
|人 ING5 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG15462-ACR|
|人 ING5 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG15462-ANG|
|人 ING5 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG15462-ANR|
|人 ING5 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG15462-CF|
|人 ING5 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG15462-CH|
|人 ING5 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG15462-CM|
|人 ING5 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG15462-CY|
|人 ING5 基因ORF全长cDNA(克隆载体)||HG15462-G|
|人 ING5 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG15462-NF|
|人 ING5 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG15462-NH|
|人 ING5 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG15462-NM|
|人 ING5 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG15462-NY|
|人 ING5 基因ORF全长cDNA克隆(表达载体)||HG15462-UT|
ING5 belongs to the ING family. It contains 1 PHD-type zinc finger and is a component of the HBO1 complex. HBO1 complex has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of PHF15, PHF16 and PHF17. ING5 also is a component of the MOZ/MORF complex which is composed at least of ING5, KAT6A, KAT6B, MEAF6 and one of BRPF1, BRD1/BRPF2 and BRPF3. It interacts with EP300 and TP53. MOZ/MORF complex has a histone H3 acetyltransferase activity. Through chromatin acetylation ING5 may regulate DNA replication and may function as a transcriptional coactivator. It interacts with H3K4me3 and to a lesser extent with H3K4me2. ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. It can bind TP EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway.