|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 LILRA3/CD85e/ILT6 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG13549-ACG|
|人 LILRA3/CD85e/ILT6 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG13549-ACR|
|人 LILRA3/CD85e/ILT6 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG13549-CF|
|人 LILRA3/CD85e/ILT6 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG13549-CH|
|人 LILRA3/CD85e/ILT6 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG13549-CM|
|人 LILRA3/CD85e/ILT6 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG13549-CY|
|人 LILRA3/CD85e/ILT6 基因ORF全长cDNA(克隆载体)||HG13549-G|
|人 LILRA3/CD85e/ILT6 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG13549-NF|
|人 LILRA3/CD85e/ILT6 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG13549-NH|
|人 LILRA3/CD85e/ILT6 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG13549-NM|
|人 LILRA3/CD85e/ILT6 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG13549-NY|
|人 LILRA3/CD85e/ILT6 基因ORF全长cDNA克隆(表达载体)||HG13549-UT|
ILT6, also known as LILRA3, belongs to the ILT family. In human, the ILT gene family includes up to 11 members. The extracellular portion of all members includes at least two and usually four immuno-globulin domains. ILT-2 through 5 are all inhibitory members having variable numbers of cytoplasmic ITIM domains. ILT6 lacks a transmembrane domain. The function of ILT6 is currently unknown. however it is highly homologous to other LILR genes, and can bind human leukocyte antigen (HLA) class I. Therefore, if secreted, the ILT6 might impair interactions of membrane-bound LILRs (such as LILRB1, an inhibitory receptor expressed on effector and memory CD8 T cells) with their HLA ligands, thus modulating immune reactions and influencing susceptibility to disease.