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人 LIPG 基因ORF全长cDNA克隆(表达载体), N-Myc 标签

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Human LIPG cDNA Clone产品信息
NCBI注册码:BC060825
参考序列ORF长度:1503bp
cDNA基因描述:Full length Clone DNA of Homo sapiens lipase, endothelial with N terminal Myc tag.
分子别称:EL, EDL, PRO719, LIPG
分子种属:Human
载体:pCMV3-SP-N-Myc
质粒:pCMV3-Myc-LIPG
限制性酶切位点:KpnI + XbaI (6kb + 1.54kb)
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human LIPG 基因质粒图
Human LIPG ORF mammalian expression plasmid, N-Myc tag
Myc Tag Info

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

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  • Human LIPG ORF mammalian expression plasmid, N-Myc tag
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