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人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), N-Flag 标签

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 LTA4H cDNA Clone产品信息
NCBI注册码:NM_000895.2
参考序列ORF长度:1836bp
cDNA基因描述:Full length Clone DNA of Homo sapiens leukotriene A4 hydrolase with N terminal Flag tag.
分子别称:LTA4H
分子种属:Human
载体:pCMV3-N-FLAG
质粒:
限制性酶切位点:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with LTA4H qPCR primers for gene expression analysis, HP100323 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), N-Flag 标签 on other vectors
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG10276-ACG2070
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG10276-ACR2070
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG10276-ANG2070
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG10276-ANR2070
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10276-CF1890
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), C-His 标签HG10276-CH1890
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG10276-CM1890
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG10276-CY1890
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA(克隆载体)HG10276-G920
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体)HG10276-G-N1890
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG10276-NF1890
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), N-His 标签HG10276-NH1890
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG10276-NM1890
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG10276-NY1890
人 Leukotriene A4 Hydrolase/LTA4H 基因ORF全长cDNA克隆(表达载体)HG10276-UT1890
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Product nameProduct name
研究背景

Mouse leukotriene A-4 hydrolase, also known as LTA-4 hydrolase, Leukotriene A (4) hydrolase, LTA4H and LTA4, is cytoplasm protein which belongs to the peptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs, arachidonic acid was converted into the unstable intermediate epoxide LTA4, which may in turn be conjugated with glutathione to form the spasmogenic LTC4, or hydrolyzed into the proinflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium, and also modulates immune responses. As a bifunctional zinc metalloenzyme, LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.

参考资料
  • Mancini, JA. et al.,1995, Eur. J. Biochem. 231: 65-71.
  • Orning, L. et al., 1994, J. Biol. Chem. 269: 11269-73.
  • Rudberg, PC. et al., 2004, J. Biol. Chem. 279: 27376-82.
  • Qiu, H. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 8161-6.
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    货号: HG10276-NF
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