|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 Lumican 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG11640-ACG|
|人 Lumican 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG11640-ACR|
|人 Lumican 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG11640-CF|
|人 Lumican 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG11640-CH|
|人 Lumican 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG11640-CM|
|人 Lumican 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG11640-CY|
|人 Lumican 基因ORF全长cDNA(克隆载体)||HG11640-M|
|人 Lumican 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG11640-NF|
|人 Lumican 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG11640-NH|
|人 Lumican 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG11640-NM|
|人 Lumican 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG11640-NY|
|人 Lumican 基因ORF全长cDNA克隆(表达载体)||HG11640-UT|
Lumican, a prototypic leucine-rich proteoglycan with keratan sulfate side chains, is a major component of the cornea, dermal, and muscle connective tissues. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and is also present in the connective tissues of other organs and embryonic corneal stroma as a glycoprotein. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. lumican expressed in injured epithelium may modulate cell behavior such as adhesion or migration, thus contributing to corneal epithelial wound healing. Lumican plays a crucial role in the regulation of collagen assembly into fibrils in various connective tissues and serve as a definitive link between a necessity for lumican in the development of a highly organized collagenous matrix and corneal transparency.