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人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签

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Human MAPK8 cDNA Clone产品信息
NCBI注册码:NM_139047.1
参考序列ORF长度:1284bp
cDNA基因描述:Full length Clone DNA of Homo sapiens mitogen-activated protein kinase 8, transcript variant JNK1-b2 with N terminal Myc tag.
分子别称:JNK, JNK1, PRKM8, SAPK1, JNK1A2, JNK21B1/2
分子种属:Human
载体:pCMV3-N-Myc
质粒:
限制性酶切位点:
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签 on other vectors
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG10795-ACG1950
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG10795-ACR1950
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG10795-ANG1950
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG10795-ANR1950
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10795-CF1770
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), C-His 标签HG10795-CH1770
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG10795-CM1770
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG10795-CY1770
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA(克隆载体)HG10795-M760
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10795-M-F1770
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体)HG10795-M-N1770
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG10795-NF1770
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), N-His 标签HG10795-NH1770
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG10795-NM1770
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG10795-NY1770
人 JNK1 / MAPK8 transcript variant JNK1-b2 基因ORF全长cDNA克隆(表达载体)HG10795-UT1770
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研究背景

Mitogen-activated protein kinase 8 (MAPK8), also known as JNK1, is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The protein kinases JNK1 has been found to serve as critical molecular links between obesity, metabolic inflammation, and disorders of glucose homeostasis. It is critically involved in the promotion of diet-induced obesity, metabolic inflammation and beta-cell dysfunction. The selective deficiency of JNK1 in the murine nervous system is sufficient to suppress diet-induced obesity. Genetic analysis indicates that the effects of JNK1 can be separated from effects of JNK1 on obesity. JNK1 is a potential pharmacological target for the development of drugs that might be useful for the treatment of metabolic syndrome, and type 2 diabetes. Furthermore, JNK1 plays a major role in the hypoxic cellular damage. JNK1 protein might be an attractive target for antihypoxic therapy in increasing resistance to many pathological conditions and diseases, leading to the oxygen deficit.

参考资料
  • Betigeri S, et al. (2006) JNK1 as a molecular target to limit cellular mortality under hypoxia. Mol Pharm. 3(4): 424-30.
  • Solinas G, et al. (2010) JNK1 and IKKbeta: molecular links between obesity and metabolic dysfunction. FASEB J. 24(8): 2596-611.
  • Sabio G, et al. (2010) Role of the hypothalamic-pituitary-thyroid axis in metabolic regulation by JNK1. Genes Dev. 24(3): 256-64.
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