The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
储存 & 运输
Lyophilized qPCR primer mix is shipped at ambiente temperatura
The lyophilized product is stable for one year from date of receipt when stored at -20℃.
The suspended product is stable for six months from date of receipt when stored at -20℃.
***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***
在特定基因的不同变体保守区内设计引物，该对引物至少有一条引物跨越内含子或产物跨越内含子，有效避免基因组 DNA 的扩增。
用质粒标准品对 qPCR 引物的灵敏度、扩增效率和特异性进行筛选，用阳性组织或细胞验证确认。
统一的 PCR 条件，操作方便，节约时间与成本
~100% 的扩增效率，保证 RNA 定量的准确性
c-MET qPCR Primer Pairs, Human: 经验证的图片
MET dissolution curves
MET amplification curves
c-MET qPCR Primer Pairs, Human: 别称
AUTS9 qPCR Primer Pairs, Human; c-Met qPCR Primer Pairs, Human; DFNB97 qPCR Primer Pairs, Human; HGFR qPCR Primer Pairs, Human; RCCP2 qPCR Primer Pairs, Human
Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.