MMP-10/MMP10 cDNA ORF Clone, Human, N-DDK (Flag®) tag

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MMP-10/MMP10 cDNA ORF Clone, Human, N-DDK (Flag®) tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
1431 bp
产品特征
Full length Clone DNA of Human matrix metallopeptidase 10 (stromelysin 2) with N terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

MMP-10/MMP10 cDNA ORF Clone, Human, N-DDK (Flag®) tag: 验证图片

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

MMP-10/MMP10 cDNA ORF Clone, Human, N-DDK (Flag®) tag: 别称

SL-2 cDNA ORF Clone, Human; STMY2 cDNA ORF Clone, Human
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