MMP-3 cDNA ORF Clone, Human, C-His tag

Cat: HG10467-CH
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MMP-3 cDNA ORF Clone, Human, C-His tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
1479 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 288T/C,306C/G,1086T/C not causing the amino acid variation.
描述
Full length Clone DNA of Human matrix metallopeptidase 3 (stromelysin 1, progelatinase) with C terminal His tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-His
限制性酶切位点
KpnI(two restriction sites) + NotI(6kb+0.83kb+0.67kb)
标签序列
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
MMP-3 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

MMP-3 cDNA ORF Clone, Human, C-His tag Validated Images
Human MMP-3 Gene Plasmid Map 17474
MMP-3 cDNA ORF Clone, Human, C-His tag Alternative Names
CHDS6 cDNA ORF Clone, Human;MMP-3 cDNA ORF Clone, Human;SL-1 cDNA ORF Clone, Human;STMY cDNA ORF Clone, Human;STMY1 cDNA ORF Clone, Human;STR1 cDNA ORF Clone, Human
MMP-3 Background Information

Matrix metallopeptidase 3 (abbreviated as MMP3) is also known as stromelysin 1 and progelatinase. MMP3 is a member of the matrix metalloproteinase (MMP) family whose members are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes including arthritis and metastasis. As a secreted zinc-dependent endopeptidase, MMP3 exerts its functions mainly in extracellular matrix. This protein is activated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMP3 plays a central role in degrading collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP3 can also active other MMPs such as MMP1, MMP7, and MMP9, rendering MMP3 crucial in connective tissue remodeling. Dysregulatoin of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Synthetic or natural inhibitors of MMPs result in inhibition of metastasis, while up-regulation of MMPs led to enhanced cancer cell invasion.

Full Name
matrix metallopeptidase 3
References
  • Sternlicht MD, et al. 1999, The stromal proteinase MMP3/stromelysin-1 promotes mammary carcinogenesis. Cell. 98(2): 137-46.
  • Yoon S, et al. (1999) Genetic analysis of MMP3, MMP9, and PAI-1 in Finnish patients with abdominal aortic or intracranial aneurysms. Biochem Biophys Res Commun. 265(2): 563-8.
  • Biondi ML, et al. (2000) MMP1 and MMP3 polymorphisms in promoter regions and cancer. Clin Chem. 46(12): 2023-4.
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