ARD1A cDNA ORF Clone, Human, N-DDK (Flag®) tag

Cat: HG14293-NF

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ARD1A cDNA ORF Clone, Human, N-DDK (Flag®) tag 基本信息

基因

种属
Human
NCBI 参考序列号
参考序列ORF长度
708 bp
描述
Full length Clone DNA of Human N(alpha)-acetyltransferase 10, NatA catalytic subunit with N terminal Flag tag.

质粒

启动子
Enhanced CMV promoter
载体
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.

筛选

抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression

储存 & 运输

运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

ARD1A cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names

ARD1 cDNA ORF Clone, Human;ARD1A cDNA ORF Clone, Human;ARD1P cDNA ORF Clone, Human;DXS707 cDNA ORF Clone, Human;MCOPS1 cDNA ORF Clone, Human;NAA10 cDNA ORF Clone, Human;NATD cDNA ORF Clone, Human;OGDNS cDNA ORF Clone, Human;TE2 cDNA ORF Clone, Human

ARD1A Background Information

ARD1 is a member of the 20-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 403-574), and a 46-kDa N-terminal domain (amino acids 1-402). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating type locus HML, for sporulation, and for entry into G0. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-110) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.

Full Name
N(alpha)-acetyltransferase 10, NatA catalytic subunit
References
  • Tribioli C., et al.,(1994), Isolation of new genes in distal Xq28: transcriptional map and identification of a human homologue of the ARD1 N-acetyl transferase of Saccharomyces cerevisiae. Hum. Mol. Genet. 3:1061-1068.
  • Arnesen T., et al., (2005), Identification and characterization of the human ARD1-NATH protein acetyltransferase complex.Biochem. J. 386:433-443.
  • Ross M.T., et al.,(2005), The DNA sequence of the human X chromosome.Nature 434:325-337.
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