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人 Alpha-galactosidase B 基因ORF全长cDNA克隆(表达载体), N-His 标签

  • Human CNDP1 Gene Plasmid Map 5655
产品数据评论相关产品实验方法
 NAGA cDNA Clone产品信息
NCBI注册码:NM_000262.2
参考序列ORF长度:1278 bp
cDNA基因描述:Full length Clone DNA of Homo sapiens N-acetylgalactosaminidase, alpha with N terminal His tag.
分子别称:GALB, D22S674, NAGA
分子种属:Human
载体:pCMV3-SP-N-His
质粒:pCMV3-SP-His-NAGA
限制性酶切位点:HindIII + NotI(6kb+1.28kb)
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列信息:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 321G/A not causing the amino acid variation.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with NAGA qPCR primers for gene expression analysis, HP102365 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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货号: HG13686-NH
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