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人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体)

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Human NEK3 cDNA Clone产品信息
NCBI注册码:NM_152720.1
参考序列ORF长度:1521bp
cDNA基因描述:Full length Clone DNA of Homo sapiens NIMA (never in mitosis gene a)-related kinase 3, transcript variant 2.
分子别称:HSPK36, MGC29949, NEK3
分子种属:Human
载体:pCMV3-untagged
质粒:pCMV3-NEK3
限制性酶切位点:KpnI + XbaI (6.1kb + 1.52kb)
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence except for the point mutation 1236T/G not causing the amino acid variation.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Ampicilin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
Human NEK3 基因质粒图
Human NEK3 transcript variant 2 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体) on other vectors
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG10675-ACG2070
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG10675-ACR2070
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG10675-ANG2070
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG10675-ANR2070
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10675-CF1890
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-His 标签HG10675-CH1890
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG10675-CM1890
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG10675-CY1890
人 NEK3 transcript variant 2 基因ORF全长cDNA(克隆载体)HG10675-M920
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10675-M-F1890
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG10675-NF1890
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-His 标签HG10675-NH1890
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG10675-NM1890
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG10675-NY1890
人 NEK3 transcript variant 2 基因ORF全长cDNA克隆(表达载体)HG10675-UT1890
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研究背景

NEK3 (NIMA (never in mitosis gene a)-related expressed kinase 3), contains 1 protein kinase domain and is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. Members of the NEK family of protein kinases share high amino acid homology with NIMA (never in mitosis gene a). NEK3 differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. It is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. NEK3 mRNA can be detected in all the proliferating cell lines with the amount not changing during the cell cycle. Prolactin stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens. Multiple transcript variants encoding different isoforms have been found for NEK3 gene. NEK3 may play a role in mitotic regulation.

参考资料
  • Miller SL, et al. (2007) Nek3 kinase regulates prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells. Oncogene. 26(32):4668-78.
  • Hernandez M, et al. (2006) Is there any association between nek3 and cancers with frequent 13q14 deletion?. Cancer Invest. 24(7):682-8.
  • Tanaka K, et al. (1999) Cloning and characterization of the murine Nek3 protein kinase, a novel member of the NIMA family of putative cell cycle regulators. J Biol Chem. 274(19):13491-7.
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    • Human NEK3 transcript variant 2 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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