( We provide with NMNAT1 qPCR primers for gene expression analysis, HP101325 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 NMNAT1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG11448-ACG|
|人 NMNAT1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG11448-ACR|
|人 NMNAT1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG11448-ANG|
|人 NMNAT1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG11448-ANR|
|人 NMNAT1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG11448-CF|
|人 NMNAT1 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG11448-CH|
|人 NMNAT1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG11448-CM|
|人 NMNAT1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG11448-CY|
|人 NMNAT1 Gene cDNA clone plasmid||HG11448-M|
|人 NMNAT1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG11448-NF|
|人 NMNAT1 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG11448-NH|
|人 NMNAT1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG11448-NM|
|人 NMNAT1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG11448-NY|
|人 NMNAT1 基因ORF全长cDNA克隆(表达载体)||HG11448-UT|
NMNAT, also known as NMNAT1, is a member of the Nicotinamide-nucleotide adenylyltransferases. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. NMNAT appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT enzyme is vital for NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD.