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人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签

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     NPM1 cDNA Clone产品信息
    NCBI注册码:NM_002520
    参考序列ORF长度:885bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens nucleophosmin (nucleolar phosphoprotein B23, numatrin) with C terminal HA tag.
    分子别称:B23, NPM
    分子种属:Human
    载体:pCMV3-C-HA
    质粒:
    限制性酶切位点:
    Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with NPM1 qPCR primers for gene expression analysis, HP101603 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签 on other vectors
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG12074-ACG1950
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG12074-ACR1950
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG12074-ANG1950
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG12074-ANR1950
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG12074-CF1770
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-His 标签HG12074-CH1770
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG12074-CM1770
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG12074-CY1770
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA(克隆载体)HG12074-G760
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG12074-NF1770
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-His 标签HG12074-NH1770
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG12074-NM1770
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG12074-NY1770
    人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体)HG12074-UT1770
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    Product nameProduct name
    研究背景

    Nucleophosmin 1 (NPM1), also known as nucleolar phosphoprotein B23 or numatrin, is a member of the nucleoplasmin family. Nucleophosmin (NPM) is a nucleolar phosphoprotein that plays multiple roles in ribosome assembly and transport, cytoplasmic-nuclear trafficking, centrosome duplication and regulation of p53. The NPM1 gene is frequently involved in chromosomal translocation, mutation and deletion. Mutations of the NPM1 gene leading to the expression of a cytoplasmic mutant protein, NPMc+, are the most frequent genetic abnormalities found in acute myeloid leukemias. Acute myeloid leukemias (AML) with mutated NPM1 have distinct characteristics, including a significant association with a normal karyotype, involvement of different hematopoietic lineages, a specific gene-expression profile and clinically, a better response to induction therapy and a favorable prognosis. In addition, NPM1 is a crucial gene to consider in the context of the genetics and biology of cancer. NPM1 is frequently overexpressed, mutated, rearranged and deleted in human cancer. Traditionally regarded as a tumour marker and a putative proto-oncogene, it has now also been attributed with tumour-suppressor functions.

    参考资料
  • Chen W, et al. (2006) Nucleophosmin gene mutations in acute myeloid leukemia. Arch Pathol Lab Med. 130(11): 1687-92.
  • Naoe T, et al. (2006) Nucleophosmin: a versatile molecule associated with hematological malignancies. Cancer Sci. 97(10): 963-9.
  • Grisendi S, et al. (2006) Nucleophosmin and cancer. Nat Rev Cancer. 6(7): 493-505.
  • Falini B, et al. (2007) Acute myeloid leukemia carrying cytoplasmic/mutated nucleophosmin (NPMc+ AML): biologic and clinical features. Blood. 109(3): 874-85.
  • Meani N, et al. (2009) Role of nucleophosmin in acute myeloid leukemia. Expert Rev Anticancer Ther. 9(9): 1283-94.
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    货号: HG12074-CY
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