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人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签

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Human NPM1 cDNA Clone产品信息
NCBI注册码:NM_002520
参考序列ORF长度:885bp
cDNA基因描述:Full length Clone DNA of Homo sapiens nucleophosmin (nucleolar phosphoprotein B23, numatrin) with N terminal Flag tag.
分子别称:B23, NPM
分子种属:Human
载体:pCMV3-N-FLAG
质粒:
限制性酶切位点:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签 on other vectors
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG12074-ACG1950
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG12074-ACR1950
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG12074-ANG1950
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG12074-ANR1950
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG12074-CF1770
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-His 标签HG12074-CH1770
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG12074-CM1770
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG12074-CY1770
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA(克隆载体)HG12074-G760
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG12074-NF1770
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-His 标签HG12074-NH1770
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG12074-NM1770
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG12074-NY1770
人 NPM1/Nucleophosmin transcript variant 1 基因ORF全长cDNA克隆(表达载体)HG12074-UT1770
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Product nameProduct name
研究背景

Nucleophosmin 1 (NPM1), also known as nucleolar phosphoprotein B23 or numatrin, is a member of the nucleoplasmin family. Nucleophosmin (NPM) is a nucleolar phosphoprotein that plays multiple roles in ribosome assembly and transport, cytoplasmic-nuclear trafficking, centrosome duplication and regulation of p53. The NPM1 gene is frequently involved in chromosomal translocation, mutation and deletion. Mutations of the NPM1 gene leading to the expression of a cytoplasmic mutant protein, NPMc+, are the most frequent genetic abnormalities found in acute myeloid leukemias. Acute myeloid leukemias (AML) with mutated NPM1 have distinct characteristics, including a significant association with a normal karyotype, involvement of different hematopoietic lineages, a specific gene-expression profile and clinically, a better response to induction therapy and a favorable prognosis. In addition, NPM1 is a crucial gene to consider in the context of the genetics and biology of cancer. NPM1 is frequently overexpressed, mutated, rearranged and deleted in human cancer. Traditionally regarded as a tumour marker and a putative proto-oncogene, it has now also been attributed with tumour-suppressor functions.

参考资料
  • Chen W, et al. (2006) Nucleophosmin gene mutations in acute myeloid leukemia. Arch Pathol Lab Med. 130(11): 1687-92.
  • Naoe T, et al. (2006) Nucleophosmin: a versatile molecule associated with hematological malignancies. Cancer Sci. 97(10): 963-9.
  • Grisendi S, et al. (2006) Nucleophosmin and cancer. Nat Rev Cancer. 6(7): 493-505.
  • Falini B, et al. (2007) Acute myeloid leukemia carrying cytoplasmic/mutated nucleophosmin (NPMc+ AML): biologic and clinical features. Blood. 109(3): 874-85.
  • Meani N, et al. (2009) Role of nucleophosmin in acute myeloid leukemia. Expert Rev Anticancer Ther. 9(9): 1283-94.
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