|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|人 NRAS / N-Ras 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG12073-ACG|
|人 NRAS / N-Ras 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG12073-ACR|
|人 NRAS / N-Ras 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG12073-CF|
|人 NRAS / N-Ras 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG12073-CH|
|人 NRAS / N-Ras 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG12073-CM|
|人 NRAS / N-Ras 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG12073-CY|
|人 NRAS / N-Ras 基因ORF全长cDNA(克隆载体)||HG12073-G|
|人 NRAS / N-Ras 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG12073-NF|
|人 NRAS / N-Ras 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG12073-NH|
|人 NRAS / N-Ras 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG12073-NM|
|人 NRAS / N-Ras 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG12073-NY|
|人 NRAS / N-Ras 基因ORF全长cDNA克隆(表达载体)||HG12073-UT|
NRAS was discovered by researchers at the Institute of Cancer Research, funded by the Cancer Research Campaign (now Cancer Research UK). NRAS gene is a member of the Ras gene family. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. The mammalian ras gene family consists of the harvey and kirsten ras genes (HRAS and KRAS), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 40 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. The NRAS gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The NRAS gene consists of seven exons (-I, I, II, III, IV, V, VI).