|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 NRG4 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG12183-ACG|
|人 NRG4 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG12183-ACR|
|人 NRG4 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG12183-CF|
|人 NRG4 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG12183-CH|
|人 NRG4 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG12183-CM|
|人 NRG4 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG12183-CY|
|人 NRG4 基因ORF全长cDNA(克隆载体)||HG12183-G|
|人 NRG4 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG12183-NF|
|人 NRG4 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG12183-NH|
|人 NRG4 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG12183-NM|
|人 NRG4 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG12183-NY|
|人 NRG4 基因ORF全长cDNA克隆(表达载体)||HG12183-UT|
NRG4 (neuregulin 4) is a member of the neuregulin protein family. The neuregulins consist of four structurally-related proteins that are part of the EGF family of proteins. It has been shown that these proteins have diverse functions in the development of the nervous system and play multiple essential roles in vertebrate embryogenesis including: cardiac development, Schwann cell and oligodendrocyte differentiation, some aspects of neuronal development, as well as the formation of neuromuscular synapses. NRG4 contains 1 EGF-like domain. It activates type-1 growth factor receptors to initiating cell-to-cell signaling through tyrosine phosphorylation. NRG4 is a low affinity ligand for the ERBB4 tyrosine kinase receptor. It concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. However, it does not bind to the ERBB1, ERBB2 and ERBB3 receptors.