( We provide with PAH qPCR primers for gene expression analysis, HP101609 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 PAH / PH 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG12081-ACG|
|人 PAH / PH 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG12081-ACR|
|人 PAH / PH 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG12081-ANG|
|人 PAH / PH 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG12081-ANR|
|人 PAH / PH 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG12081-CF|
|人 PAH / PH 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG12081-CH|
|人 PAH / PH 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG12081-CM|
|人 PAH / PH 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG12081-CY|
|人 PAH 基因全长ORF克隆||HG12081-G|
|人 PAH / PH 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG12081-NF|
|人 PAH / PH 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG12081-NH|
|人 PAH / PH 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG12081-NM|
|人 PAH / PH 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG12081-NY|
|人 PAH / PH 基因ORF全长cDNA(克隆载体)||HG12081-U|
|人 PAH / PH 基因ORF全长cDNA克隆(表达载体)||HG12081-UT|
PAH (phenylalanine hydroxylase), also known as PH, belongs to the biopterin-dependent aromatic amino acid hydroxylase family. It contains 1 ACT domain, N-terminal region of PAH is thought to contain allosteric binding sites for phenylalanine and to constitute an "inhibitory" domain that regulates the activity of a catalytic domain in the C-terminal portion of the molecule. In humans, PAH is expressed both in the liver and the kidney, and there is some indication that it may be differentially regulated in these tissues. PAH catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. It is one of three members of the pterin-dependent amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin and a non-heme iron for catalysis. Defects in PAH are the cause of phenylketonuria (PKU). PKU is an autosomal recessive inborn error of phenylalanine metabolism, due to severe phenylalanine hydroxylase deficiency. It is characterized by blood concentrations of phenylalanine persistently above 1200 mumol.