PDGF-B cDNA ORF Clone, Human, N-DDK (Flag®) tag 基本信息
基因
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) with N terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI (6kb + 0.75kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
PDGF-B cDNA ORF Clone, Human, N-DDK (Flag®) tag Validated Images
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
PDGF-B cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names
c-sis cDNA ORF Clone, Human;IBGC5 cDNA ORF Clone, Human;PDGF-2 cDNA ORF Clone, Human;PDGF2 cDNA ORF Clone, Human;SIS cDNA ORF Clone, Human;SSV cDNA ORF Clone, Human
PDGF-B Background Information
Full Name
platelet-derived growth factor beta polypeptide
