PDGF-B cDNA ORF Clone, Human, N-DDK (Flag®) tag

Cat: HG10572-NF
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PDGF-B cDNA ORF Clone, Human, N-DDK (Flag®) tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
726 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) with N terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-SP-N-FLAG
限制性酶切位点
KpnI + XbaI (6kb + 0.75kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
PDGF-B cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

PDGF-B cDNA ORF Clone, Human, N-DDK (Flag®) tag Validated Images
Rhesus CD64/Fc gamma RI Gene Plasmid Map 5614
Human PDGF-B/PDGF-2 Gene Expression validated Image 16368
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
PDGF-B cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names
c-sis cDNA ORF Clone, Human;IBGC5 cDNA ORF Clone, Human;PDGF-2 cDNA ORF Clone, Human;PDGF2 cDNA ORF Clone, Human;SIS cDNA ORF Clone, Human;SSV cDNA ORF Clone, Human
PDGF-B Background Information
Full Name
platelet-derived growth factor beta polypeptide

PDGF-B cDNA ORF Clone, Rhesus, Related Products

Product Name Catalog#(PDF)
PDGF-B cDNA ORF Clone in Cloning Vector, Rhesus CG90030-G
PDGF-B cDNA ORF Clone, Rhesus, untagged CG90030-UT
PDGF-B Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90030-ACGLN
PDGF-B cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90030-ACG
PDGF-B cDNA ORF Clone, Rhesus, C-OFPSpark® tag CG90030-ACR
PDGF-B cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90030-CF
PDGF-B cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90030-NF
PDGF-B cDNA ORF Clone, Rhesus, C-His tag CG90030-CH
PDGF-B cDNA ORF Clone, Rhesus, N-His tag CG90030-NH
PDGF-B cDNA ORF Clone, Rhesus, C-HA tag CG90030-CY
PDGF-B cDNA ORF Clone, Rhesus, N-HA tag CG90030-NY
PDGF-B cDNA ORF Clone, Rhesus, C-Myc tag CG90030-CM
PDGF-B cDNA ORF Clone, Rhesus, N-Myc tag CG90030-NM

PDGF-B cDNA ORF Clone, Canine, Related Products

Product Name Catalog#(PDF)
PDGF-B cDNA ORF Clone in Cloning Vector, Canine DG70053-G
PDGF-B cDNA ORF Clone, Canine, untagged DG70053-UT
PDGF-B Lentiviral cDNA ORF Clone, Canine, C-GFPSpark® tag DG70053-ACGLN
PDGF-B cDNA ORF Clone, Canine, C-GFPSpark® tag DG70053-ACG
PDGF-B cDNA ORF Clone, Canine, C-OFPSpark® tag DG70053-ACR
PDGF-B cDNA ORF Clone, Canine, C-DDK (Flag®) tag DG70053-CF
PDGF-B cDNA ORF Clone, Canine, N-DDK (Flag®) tag DG70053-NF
PDGF-B cDNA ORF Clone, Canine, C-His tag DG70053-CH
PDGF-B cDNA ORF Clone, Canine, N-His tag DG70053-NH
PDGF-B cDNA ORF Clone, Canine, C-HA tag DG70053-CY
PDGF-B cDNA ORF Clone, Canine, N-HA tag DG70053-NY
PDGF-B cDNA ORF Clone, Canine, C-Myc tag DG70053-CM
PDGF-B cDNA ORF Clone, Canine, N-Myc tag DG70053-NM

PDGF-B cDNA ORF Clone, Rat, Related Products

Product Name Catalog#(PDF)
PDGF-B cDNA ORF Clone in Cloning Vector, Rat RG80053-M
PDGF-B cDNA ORF Clone, Rat, untagged RG80053-UT
PDGF-B cDNA ORF Clone, Rat, C-GFPSpark® tag RG80053-ACG
PDGF-B Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80053-ACGLN
PDGF-B cDNA ORF Clone, Rat, C-OFPSpark® tag RG80053-ACR
PDGF-B cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80053-CF
PDGF-B cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80053-NF
PDGF-B cDNA ORF Clone, Rat, C-His tag RG80053-CH
PDGF-B cDNA ORF Clone, Rat, N-His tag RG80053-NH
PDGF-B cDNA ORF Clone, Rat, C-HA tag RG80053-CY
PDGF-B cDNA ORF Clone, Rat, N-HA tag RG80053-NY
PDGF-B cDNA ORF Clone, Rat, C-Myc tag RG80053-CM
PDGF-B cDNA ORF Clone, Rat, N-Myc tag RG80053-NM
PDGF-B qPCR Primer Pairs, Rat RP300051

PDGF-B cDNA ORF Clone, Mouse, Related Products

Product Name Catalog#(PDF)
PDGF-B cDNA ORF Clone in Cloning Vector, Mouse MG50801-G
PDGF-B cDNA ORF Clone, Mouse, untagged MG50801-UT
PDGF-B cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50801-ACG
PDGF-B Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50801-ACGLN
PDGF-B cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50801-ACR
PDGF-B cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50801-CF
PDGF-B cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50801-NF
PDGF-B cDNA ORF Clone, Mouse, C-His tag MG50801-CH
PDGF-B cDNA ORF Clone, Mouse, N-His tag MG50801-NH
PDGF-B cDNA ORF Clone, Mouse, C-HA tag MG50801-CY
PDGF-B cDNA ORF Clone, Mouse, N-HA tag MG50801-NY
PDGF-B cDNA ORF Clone, Mouse, C-Myc tag MG50801-CM
PDGF-B cDNA ORF Clone, Mouse, N-Myc tag MG50801-NM
PDGF-B qPCR Primer Pairs, Mouse MP200772

PDGF-B cDNA ORF Clone, Human, Related Products

Product Name Catalog#(PDF)
PDGF-B cDNA ORF Clone in Cloning Vector, Human HG10572-M
PDGF-B cDNA ORF Clone, Human, untagged HG10572-UT
PDGF-B Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10572-ACGLN
PDGF-B cDNA ORF Clone, Human, C-GFPSpark® tag HG10572-ACG
PDGF-B cDNA ORF Clone, Human, C-OFPSpark® tag HG10572-ACR
PDGF-B cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10572-CF
PDGF-B cDNA ORF Clone, Human, C-His tag HG10572-CH
PDGF-B cDNA ORF Clone, Human, N-His tag HG10572-NH
PDGF-B cDNA ORF Clone, Human, C-HA tag HG10572-CY
PDGF-B cDNA ORF Clone, Human, N-HA tag HG10572-NY
PDGF-B cDNA ORF Clone, Human, C-Myc tag HG10572-CM
PDGF-B cDNA ORF Clone, Human, N-Myc tag HG10572-NM
PDGF-B qPCR Primer Pairs, Human HP100579
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