PFKL cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG14223-CF
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PFKL cDNA ORF Clone, Human, C-DDK (Flag®) tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
2343 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human phosphofructokinase, liver with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-FLAG
限制性酶切位点
HindIII + XbaI (6kb + 2.38kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
PFKL cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

PFKL cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images
Cynomolgus CD19/B4/CVID3 Gene Plasmid Map 5619
Human PFKL Gene Expression validated Image 14369
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
PFKL cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
ATP-PFK cDNA ORF Clone, Human;PFK-B cDNA ORF Clone, Human;PFK-L cDNA ORF Clone, Human
PFKL Background Information
Full Name
phosphofructokinase, liver

PFKL cDNA ORF Clone, Mouse, Related Products

Product Name Catalog#(PDF)
PFKL cDNA ORF Clone in Cloning Vector, Mouse MG52702-G
PFKL cDNA ORF Clone, Mouse, untagged MG52702-UT
PFKL cDNA ORF Clone, Mouse, C-GFPSpark® tag MG52702-ACG
PFKL Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG52702-ACGLN
PFKL cDNA ORF Clone, Mouse, N-GFPSpark® tag MG52702-ANG
PFKL cDNA ORF Clone, Mouse, C-OFPSpark® tag MG52702-ACR
PFKL cDNA ORF Clone, Mouse, N-OFPSpark® tag MG52702-ANR
PFKL cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG52702-CF
PFKL cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG52702-NF
PFKL cDNA ORF Clone, Mouse, C-His tag MG52702-CH
PFKL cDNA ORF Clone, Mouse, N-His tag MG52702-NH
PFKL cDNA ORF Clone, Mouse, C-HA tag MG52702-CY
PFKL cDNA ORF Clone, Mouse, N-HA tag MG52702-NY
PFKL cDNA ORF Clone, Mouse, C-Myc tag MG52702-CM
PFKL cDNA ORF Clone, Mouse, N-Myc tag MG52702-NM
PFKL qPCR Primer Pairs, Mouse MP202573

PFKL cDNA ORF Clone, Human, Related Products

Product Name Catalog#(PDF)
PFKL cDNA ORF Clone in Cloning Vector, Human HG14223-G
PFKL cDNA ORF Clone, Human, untagged HG14223-UT
PFKL Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG14223-ACGLN
PFKL cDNA ORF Clone, Human, C-GFPSpark® tag HG14223-ACG
PFKL cDNA ORF Clone, Human, N-GFPSpark® tag HG14223-ANG
PFKL cDNA ORF Clone, Human, C-OFPSpark® tag HG14223-ACR
PFKL cDNA ORF Clone, Human, N-OFPSpark® tag HG14223-ANR
PFKL cDNA ORF Clone, Human, N-DDK (Flag®) tag HG14223-NF
PFKL cDNA ORF Clone, Human, C-His tag HG14223-CH
PFKL cDNA ORF Clone, Human, N-His tag HG14223-NH
PFKL cDNA ORF Clone, Human, C-HA tag HG14223-CY
PFKL cDNA ORF Clone, Human, N-HA tag HG14223-NY
PFKL cDNA ORF Clone, Human, C-Myc tag HG14223-CM
PFKL cDNA ORF Clone, Human, N-Myc tag HG14223-NM
PFKL qPCR Primer Pairs, Human HP102873
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