|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 PLBD2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG13947-ACG|
|人 PLBD2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG13947-ACR|
|人 PLBD2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG13947-CF|
|人 PLBD2 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG13947-CH|
|人 PLBD2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG13947-CM|
|人 PLBD2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG13947-CY|
|人 PLBD2 基因ORF全长cDNA(克隆载体)||HG13947-G|
|人 PLBD2 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG13947-G-H|
|人 PLBD2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG13947-NF|
|人 PLBD2 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG13947-NH|
|人 PLBD2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG13947-NM|
|人 PLBD2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG13947-NY|
|人 PLBD2 基因ORF全长cDNA克隆(表达载体)||HG13947-UT|
PLBD2 localizes to the lysosome, as its absence could plausibly lead to a serious yet unrecognized lysosomal storage disease. PLBD1 and PLBD2 are semi-orphans in the sense of being probable phospholipases of B class but with uncertain physiological substrates and thus functionalities. PLBD1 and PLBD2 constitute a small gene family (sequence homology class) within vertebrates though one that occurs expanded in some early diverging eukaryotes. PLBD2 presents a special difficulty in that a sequence of post-translational steps are apparently necessary for its activation. Without these, potential substrates can hardly be assayed. These steps include removal of the signal peptide, mannosylation appropriate to the lysosome targeting receptor, and self-catalytic proteolytic activation to expose the substrate binding site as this becomes appropriate.