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人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), N-HA 标签

产品数据评论相关产品实验方法
Human PMM2 cDNA Clone产品信息
NCBI注册码:BC008310
参考序列ORF长度:741bp
cDNA基因描述:Full length Clone DNA of Homo sapiens phosphomannomutase 2 with N terminal HA tag.
分子别称:PMI, CDG1, CDGS, PMI1, CDG1a, PMM 2
分子种属:Human
载体:pCMV3-N-HA
质粒:
限制性酶切位点:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), N-HA 标签 on other vectors
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG14648-ACG1950
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG14648-ACR1950
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG14648-ANG1950
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG14648-ANR1950
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG14648-CF1770
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), C-His 标签HG14648-CH1770
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG14648-CM1770
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG14648-CY1770
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA(克隆载体)HG14648-G760
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG14648-NF1770
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), N-His 标签HG14648-NH1770
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG14648-NM1770
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG14648-NY1770
人 phosphomannomutase 2 / PMM2 / CDG1 基因ORF全长cDNA克隆(表达载体)HG14648-UT1770
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研究背景

Phosphomannomutase 2, also known as PMM2 and CDG1, belongs to the eukaryotic PMM family. Phosphomannomutase 2 catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate. Mannose 1-phosphate is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. GDP-mannose can transfer its small sugar molecule called mannose to the growing oligosaccharide chain. Once the correct number of small sugar molecules are linked together to form the oligosaccharide, it can be attached to a protein. Phosphomannomutase 2 is also required for a number of critical mannosyl transfer reactions. Mutations in PMM2 gene have been shown to cause defects in the protein glycosylation pathway manifest as carbohydrate-deficient glycoprotein syndrome type I.

参考资料
  • Jaeken J. et al., 2002, Annual review of genomics and human genetics. 2: 129-51.
  • Matthijs G. et al., 2000, Mol Genet Metab. 68 (2): 220-6.
  • Matthijs G. et al., 1997, Nat Genet. 16 (1): 88-92.
  • Size / Price
    货号: HG14648-NY
    目录价: 
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    库存2-3 weeks
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