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人 PPARG 基因ORF全长cDNA克隆(表达载体), N-His 标签

  • Ferret TNF-alpha/TNFA/TNFSF2 Gene Plasmid Map 5629
产品数据评论相关产品实验方法
 PPARG cDNA Clone产品信息
NCBI注册码:NM_015869.4
参考序列ORF长度:1563 bp
cDNA基因描述:Full length Clone DNA of Homo sapiens peroxisome proliferator-activated receptor gamma with N terminal His tag.
分子别称:PPARG1, PPARG2, NR1C3, PPARgamma
分子种属:Human
载体:pCMV3-N-His
质粒:pCMV3-His-PPARG
限制性酶切位点:KpnI + XbaI(pCMV3-N-His-KpnI-XbaI)
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with PPARG qPCR primers for gene expression analysis, HP101555 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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参考资料
  • Hamblin M, et al. (2009) PPARs and the Cardiovascular System. Antioxid Redox Signal. 11 (6): 1415-52.
  • Khateeb J, et al. (2010) Paraoxonase 1 (PON1) expression in hepatocytes is upregulated by pomegranate polyphenols: a role for PPAR-gamma pathway. Atherosclerosis. 208 (1): 119-25.
  • Fajas L, et al. (1997) The organization, promoter analysis, and expression of the human PPARgamma gene. J Biol Chem. 272 (30): 18779-89.
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    货号: HG12019-NH
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