|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 PPM1G 基因全长ORF克隆 (表达载体), C端GFPSpark标签||HG11245-ACG|
|人 PPM1G 基因全长ORF克隆 (表达载体), C端OFPSpark标签||HG11245-ACR|
|人 PPM1G 基因全长ORF克隆 (表达载体), N端GFPSpark标签||HG11245-ANG|
|人 PPM1G 基因全长ORF克隆 (表达载体), N端OFPSpark标签||HG11245-ANR|
|人 PPM1G 基因全长ORF克隆 (表达载体), C端FLAG标签||HG11245-CF|
|人 PPM1G 基因全长ORF克隆 (表达载体), C端His标签||HG11245-CH|
|人 PPM1G 基因全长ORF克隆 (表达载体), C端Myc标签||HG11245-CM|
|人 PPM1G 基因全长ORF克隆 (表达载体), C端HA标签||HG11245-CY|
|人 PPM1G 基因全长ORF克隆||HG11245-M|
|人 PPM1G 基因全长ORF克隆 (表达载体), N端FLAG标签||HG11245-NF|
|人 PPM1G 基因全长ORF克隆 (表达载体), N端His标签||HG11245-NH|
|人 PPM1G 基因全长ORF克隆 (表达载体), N端Myc标签||HG11245-NM|
|人 PPM1G 基因全长ORF克隆 (表达载体), N端HA标签||HG11245-NY|
|人 PPM1G 基因全长ORF克隆 (表达载体), 无标签||HG11245-UT|
Protein phosphatase 1G, also known as Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma, PPM1G and PPM1C, is a cytoplasm protein which belongs to the PP2C family. PPM1G / PP2C-gamma is widely expressed. It is most abundant in testis, skeletal muscle, and heart. Alternatively spliced transcript variants encoding the same protein have been described. PP2C family members are known to be negative regulators of cell stress response pathways. PPM1G / PP2C-gamma is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. PPM1G / PP2C-gamma also plays a role in regulating cell cycle progression.