( We provide with PTP4A2 qPCR primers for gene expression analysis, HP102573 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 PRL-2/PTP4A2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG13097-ACG|
|人 PRL-2/PTP4A2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG13097-ACR|
|人 PRL-2/PTP4A2 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG13097-ANG|
|人 PRL-2/PTP4A2 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG13097-ANR|
|人 PRL-2/PTP4A2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG13097-CF|
|人 PRL-2/PTP4A2 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG13097-CH|
|人 PRL-2/PTP4A2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG13097-CM|
|人 PRL-2/PTP4A2 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG13097-CY|
|人 PRL-2/PTP4A2 基因ORF全长cDNA(克隆载体)||HG13097-G|
|人 PRL-2/PTP4A2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG13097-NF|
|人 PRL-2/PTP4A2 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG13097-NH|
|人 PRL-2/PTP4A2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG13097-NM|
|人 PRL-2/PTP4A2 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG13097-NY|
|人 PRL-2/PTP4A2 基因ORF全长cDNA克隆(表达载体)||HG13097-UT|
PRL-2 (Protein-tyrosine phosphatase of regenerating liver 2), also known as PTP4A2 (Protein tyrosine phosphatase type IVA, member 2), is a member of PTP family and has an important function in controlling cell growth. PRL-2 phosphatases may be multifunctional enzymes with diverse roles in a variety of tissue and cell types. The phosphatase of regenerating liver (PRL) family, comprising PRL-1, PRL-2 and PRL-3, is a group of prenylated phosphatases that are candidate cancer biomarkers and therapeutic targets. PRL-1, PRL-2, and PRL-3 represent a novel class of protein-tyrosine phosphatase with a C-terminal prenylation motif. They are three closely related intracellular enzymes that possess the PTP active site signature sequence CX 5R. The PRL-2 mRNA is elevated in primary breast tumors relative to matched normal tissue, and also dramatically elevated in metastatic lymph nodes compared with primary tumors. PRL-2 plays a role in breast cancer progression. PRL-2 is a pathogenic molecule in hematopoietic malignancies and suggest its potential as a novel therapeutic target.