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人 Pleiotrophin / PTN / HB-GAM 基因ORF全长cDNA克隆(表达载体)

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 PTN cDNA Clone产品信息
NCBI注册码:NM_002825.5
参考序列ORF长度:498bp
cDNA基因描述:Full length Clone DNA of Homo sapiens pleiotrophin.
分子别称:HARP, HBNF, HBGF8, NEGF1.
分子种属:Human
载体:pCMV3-untagged
质粒:pCMV3-PTN
限制性酶切位点:HindIII + XbaI (6.1kb + 0.5kb)
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with PTN qPCR primers for gene expression analysis, HP100333 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Ampicillin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
 PTN 基因质粒图
Human Pleiotrophin / PTN Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
Product nameProduct name
研究背景

HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damaged in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.

参考资料
  • Vanderwinden JM, et al. (1992) Cellular distribution of the new growth factor pleiotrophin (HB-GAM) mRNA in developing and adult rat tissues. Anat Embryol. 186(4):387-406.
  • Lauri SE, et al. (1996) Activity-induced enhancement of HB-GAM expression in rat hippocampal slices. Neuroreport. 7(10):1670-4.
  • Pavlov I, et al. (2002) Role of heparin-binding growth-associated molecule (HB-GAM) in hippocampal LTP and spatial learning revealed by studies on overexpressing and knockout mice. Mol Cell Neurosci. 20(2):330-42.
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    货号: HG10288-UT
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    • Human Pleiotrophin / PTN Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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