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人 Prostasin/PRSS8 基因ORF全长cDNA克隆(表达载体)

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 PRSS8 cDNA Clone产品信息
NCBI注册码:NM_002773.3
参考序列ORF长度:1032bp
cDNA基因描述:Full length Clone DNA of Homo sapiens protease, serine, 8.
分子别称:CAP1, PROSTASIN
分子种属:Human
载体:pCMV3-untagged
质粒:pCMV3-PRSS8
限制性酶切位点:HindIII + XbaI (6.1kb + 1.03kb)
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with PRSS8 qPCR primers for gene expression analysis, HP100332 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Ampicillin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
 PRSS8 基因质粒图
Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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研究背景

Prostasin (Prss8), also known as channel activating protease 1 (CAP1), is a trypsinlike serine peptidase, and plays important roles in epithelial physiology. It is originally purified as an active, soluble enzyme from human seminal fluid and is highly expressed in prostate, lung, kidney, salivary gland and pancreas. Prostasin is expressed as a glycosyl-phosphatidylinositol (GPI)-anchored membrane protein in prostate epithelial cells, and also exists as a secreted proteolytic enzyme possibly via tryptic cleavage of its COOH-terminal hydrophobic domain. Prostasin is found to activate the epithelial sodium channel (ENaC) which is tightly regulated and is critical for maintaining salt and fluid balance in the lung and kidney in both normal and pathological conditions. Accordingly, prostasin has been proposed as a target for therapeutic inhibition in cystic fibrosis. In addition, prostasin inhibits prostate and breast cancer cell invasion in vitro, suggesting a functional role as a suppressor of tumor invasion, as well as a regulator of gene expression during inflammation.

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货号: HG10287-UT
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  • Human Prostasin / Prss8 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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