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人 ROBO2 transcript variant 2 基因ORF全长cDNA克隆(表达载体)

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 ROBO2 cDNA Clone产品信息
NCBI注册码:NM_002942.4
参考序列ORF长度:4137bp
cDNA基因描述:Full length Clone DNA of Homo sapiens roundabout, axon guidance receptor, homolog 2 (Drosophila), transcript variant 2.
分子别称:SAX3, KIAA1568
分子种属:Human
载体:pCMV3-untagged
质粒:pCMV3-ROBO2
限制性酶切位点:HindIII (two restriction sites) + NotI (6.09kb+3.69kb+0.47kb)
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with ROBO2 qPCR primers for gene expression analysis, HP100353 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Ampicillin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
 ROBO2 基因质粒图
Human ROBO2 transcript variant 2 natural ORF mammalian expression plasmid
Product nameProduct name
研究背景

ROBO2 belongs to the ROBO family. Members of the ROBO family are a group of highly conserved transmembrane glycoproteins that make up a small subgroup of the immunoglobulin (Ig) superfamily. They are best known for their roles as receptors for the Slit family of repellent axon guidance cues. In structure, ROBOs are characterized by five C2-type Ig-like repeats, three fibronectin type III domains, a transmembrane region, and an intracellular domain with three (ROBO3) or four (ROBO1, 2) CC (conserved cytoplasmic) motifs. ROBO2 is a receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. ROBO2 also abrogates SLIT-ROBO signaling in vitro.

参考资料
  • Brose K, et al. (1999) Slit proteins bind Robo receptors and have an evolutionarily conserved role in repulsive axon guidance. Cell. 96(6):795-806.
  • Brose K, et al. (1999) Slit2-Mediated chemorepulsion and collapse of developing forebrain axons. Neuron. 22(3):463-73.
  • Nagase T, et al. (2001) Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7(4):273-81.
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    货号: HG10310-UT
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    • Human ROBO2 transcript variant 2 natural ORF mammalian expression plasmid
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