|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 RSK4 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG10147-ACG|
|人 RSK4 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG10147-ACR|
|人 RSK4 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG10147-ANG|
|人 RSK4 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG10147-ANR|
|人 RSK4 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG10147-CF|
|人 RSK4 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG10147-CH|
|人 RSK4 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG10147-CM|
|人 RSK4 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG10147-CY|
|人 RSK4 基因ORF全长cDNA(克隆载体)||HG10147-M|
|人 RSK4 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG10147-NF|
|人 RSK4 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG10147-NH|
|人 RSK4 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG10147-NM|
|人 RSK4 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG10147-NY|
|人 RSK4 基因ORF全长cDNA克隆(表达载体)||HG10147-UT|
Ribosomal protein S6 kinase alpha-6, also known as Ribosomal S6 kinase 4, 90 kDa ribosomal protein S6 kinase 6,RSK-4, RSK4 and RPS6KA6, is a protein which belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and S6 kinase subfamily. RPS6KA6 contains one AGC-kinase C-terminal domain and two protein kinase domains. RPS6KA6 forms a complex with either ERK1 or ERK2 in quiescent cells. RPS6KA6 shows a high level of homology to three isolated members of the human RSK family. RSK2 is involved in Coffin-Lowry syndrome and nonspecific MRX. The localization of RPS6KA6 in the interval that is commonly deleted in mentally retarded males together with the high degree of amino acid identity with RSK2 suggests that RPS6KA6 plays a role in normal neuronal development. Further mutation analyses in males with X-linked mental retardation must prove that the gene of RPS6KA6 is indeed a novel MRX gene. RPS6KA6 is a serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. RPS6KA6 is activated by multiple phosphorylations on threonine and serine residues.