( We provide with RRM1 qPCR primers for gene expression analysis, HP102966 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 RRM1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG14316-ACG|
|人 RRM1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG14316-ACR|
|人 RRM1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG14316-ANG|
|人 RRM1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG14316-ANR|
|人 RRM1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG14316-CF|
|人 RRM1 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG14316-CH|
|人 RRM1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG14316-CM|
|人 RRM1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG14316-CY|
|人 RRM1 基因ORF全长cDNA(克隆载体)||HG14316-G|
|人 RRM1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG14316-NF|
|人 RRM1 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG14316-NH|
|人 RRM1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG14316-NM|
|人 RRM1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG14316-NY|
|人 RRM1 基因ORF全长cDNA克隆(表达载体)||HG14316-UT|
RRM1 is a subunit of ribonucleoside-diphosphate reductase which is constituted by two subunits. Ribonucleoside-diphosphate reductase is an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. RRM1 is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. RRM1 may play a role in malignancies and disease that involve this region.