RAF1 cDNA ORF Clone, Human, untagged

Cat: HG10657-UT
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RAF1 cDNA ORF Clone, Human, untagged 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
1947 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human v-raf-1 murine leukemia viral oncogene homolog 1.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-untagged
限制性酶切位点
KpnI + XbaI (6.1kb + 1.95kb)
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Ampicillin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
RAF1 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

RAF1 cDNA ORF Clone, Human, untagged Validated Images
other Green fluorescent protein / GFP Gene Plasmid Map 5613
RAF1 cDNA ORF Clone, Human, untagged Alternative Names
c-Raf cDNA ORF Clone, Human;CMD1NN cDNA ORF Clone, Human;CRAF cDNA ORF Clone, Human;NS5 cDNA ORF Clone, Human;Raf-1 cDNA ORF Clone, Human
RAF1 Background Information

RAF1 gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
Raf-1 proto-oncogene, serine/threonine kinase
References
  • Zhang K, Chen H, Zhang B, et al. Overexpression of Raf-1 and ERK1/2 in sacral chordoma and association with tumor recurrence. International Journal of Clinical and Experimental Pathology. 2015;8(1):608-614.
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