PSGL-1 / CD162 cDNA ORF Clone, Human, C-Myc tag

Cat: HG13863-CM
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PSGL-1 / CD162 cDNA ORF Clone, Human, C-Myc tag 基本信息
基因
种属
Human
参考序列ORF长度
1287 bp
描述
Full length Clone DNA of Human selectin P ligand with C terminal Myc tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-Myc
标签序列
Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

PSGL-1 / CD162 cDNA ORF Clone, Human, C-Myc tag Alternative Names
CD162 cDNA ORF Clone, Human;CLA cDNA ORF Clone, Human;PSGL-1 cDNA ORF Clone, Human;PSGL1 cDNA ORF Clone, Human
PSGL-1 / CD162 Background Information

P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~120 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.

Full Name
selectin P ligand
References
  • 1. Sako, D. et al., 1993, Cell. 75: 1179-1186.
  • 2. Wilkins, P. P. et al., 1995, J. Biol. Chem. 270: 22677-22680.
  • 3. Frenette, P. S. et al., 2000, J. Exp. Med. 191: 1413-1422.
  • 4. Vandendries, E.R .et al., 2004, Thromb. Haemost. 92: 459-466..
  • 5. Pouyani, T. et al., 1995, Cell. 83: 333-343.
  • PSGL-1 / CD162 cDNA ORF Clone, Mouse, Related Products

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    PSGL-1 / CD162 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    PSGL-1 / CD162 cDNA ORF Clone in Cloning Vector, Human HG10490-M
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    PSGL-1 / CD162 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG13863-ACGLN
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    PSGL-1 / CD162 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10490-CF
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    PSGL-1 / CD162 cDNA ORF Clone, Human, C-His tag HG10490-CH
    PSGL-1 / CD162 cDNA ORF Clone, Human, N-His tag HG10490-NH
    PSGL-1 / CD162 cDNA ORF Clone, Human, C-HA tag HG10490-CY
    PSGL-1 / CD162 cDNA ORF Clone, Human, N-HA tag HG10490-NY
    PSGL-1 / CD162 cDNA ORF Clone, Human, C-Myc tag HG10490-CM
    PSGL-1 / CD162 cDNA ORF Clone, Human, N-Myc tag HG10490-NM
    PSGL-1 / CD162 qPCR Primer Pairs, Human HP100511
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