SEMA3A cDNA ORF Clone, Human, N-His tag

Cat: HG10758-NH
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SEMA3A cDNA ORF Clone, Human, N-His tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
2316 bp
描述
Full length Clone DNA of Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A with N terminal His tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-SP-N-His
标签序列
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

SEMA3A cDNA ORF Clone, Human, N-His tag Alternative Names
coll-1 cDNA ORF Clone, Human;COLL1 cDNA ORF Clone, Human;HH16 cDNA ORF Clone, Human;Hsema-I cDNA ORF Clone, Human;Hsema-III cDNA ORF Clone, Human;SEMA1 cDNA ORF Clone, Human;SEMAD cDNA ORF Clone, Human;SEMAIII cDNA ORF Clone, Human;SEMAL cDNA ORF Clone, Human;Semaphorin 3A cDNA ORF Clone, Human;SemD cDNA ORF Clone, Human
SEMA3A Background Information

Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 500 aa Sema domain. They are best characterized in relation to axon guidance during development of the nervous system. The functions of Semaphorins 3A (SEMA3A) are mediated primarily through binding to the Neuropilin-1 (Npn-1) and Plexin-A1 coreceptor complex. Neuropilins lack a signaling-competent cytoplasetmic domain and ensure semaphorin binding, whereas the transmembrane receptor plexin mediates the intracellular response. As the first identified vertebrate semaphorin, SEMA3A functions either as a chemorepulsive agent inhibiting axonal outgrowth, or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Its overexpression is associated with schizophrenia which is seen in various human tumor cell lines, and aberrant release is associated with the progression of Alzheimer's disease

Full Name
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
References
  • Giordano,A. et al., 2003, J Neurocytol.32(4):345-352.
  • Good, P. F. et al., 2005, J. Neurochem.91(3): 716-736.
  • Gu, C. et al., 2005, Science.307(5707): 265–268.
  • Chadborn,N.H. et al., 2006, J Cell Sci.119(Pt 5):951-957.
  • Schmidt,E.F. et al., 2007, Adv Exp Med Biol.600:1-11.
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