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人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-HA 标签

    产品数据评论相关产品实验方法
     SFN cDNA Clone产品信息
    NCBI注册码:NM_006142.3
    参考序列ORF长度:747bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens stratifin with C terminal HA tag.
    分子别称:YWHAS, SFN
    分子种属:Human
    载体:pCMV3-C-HA
    质粒:
    限制性酶切位点:
    Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with SFN qPCR primers for gene expression analysis, HP100737 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-HA 标签 on other vectors
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG10838-ACG1950
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG10838-ACR1950
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG10838-ANG1950
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG10838-ANR1950
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10838-CF1770
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-His 标签HG10838-CH1770
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG10838-CM1770
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG10838-CY1770
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA(克隆载体)HG10838-M760
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10838-M-F1770
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG10838-NF1770
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), N-His 标签HG10838-NH1770
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG10838-NM1770
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG10838-NY1770
    人 14-3-3 sigma/Stratifin/YWHAS 基因ORF全长cDNA克隆(表达载体)HG10838-UT1770
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    Product nameProduct name
    研究背景

    14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

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    货号: HG10838-CY
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    Datasheet & Documentation

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