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人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), C端FLAG标签

产品数据评论相关产品实验方法
Human DIABLO cDNA Clone产品信息
NCBI注册码:NM_019887.3
参考序列ORF长度:720bp
cDNA基因描述:Full length Clone DNA of Homo sapiens diablo homolog (Drosophila), nuclear gene encoding mitochondrial protein, transcript variant 1 with C terminal Flag tag.
分子别称:SMAC, SMAC3, DIABLO-S, FLJ10537, FLJ25049
分子种属:Human
载体:pCMV3-C-FLAG
质粒:
限制性酶切位点:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), C端FLAG标签 on other vectors
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), C端GFPSpark标签HG10339-ACG1950
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), C端OFPSpark标签HG10339-ACR1950
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), N端GFPSpark标签HG10339-ANG1950
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), N端OFPSpark标签HG10339-ANR1950
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), C端FLAG标签HG10339-CF1770
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), C端His标签HG10339-CH1770
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), C端Myc标签HG10339-CM1770
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), C端HA标签HG10339-CY1770
人 SMAC / Diablo 转录变体1 基因全长ORF克隆HG10339-M760
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), 带FLAG标签HG10339-M-F2360
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), N端FLAG标签HG10339-NF1770
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), N端His标签HG10339-NH1770
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), N端Myc标签HG10339-NM1770
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), N端HA标签HG10339-NY1770
人 SMAC / Diablo 转录变体1 基因全长ORF克隆 (表达载体), 无标签HG10339-UT1770
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研究背景

Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer.

参考资料
  • Korga A, et al. (2006) Role of mitochondrial protein Smac/Diablo in regulation of apoptotic pathways Pol Merkur Lekarski. 20(119): 573-6.
  • Anguiano-Hernandez YM, et al. (2007) Smac/DIABLO and colon cancer. Anticancer Agents Med Chem. 7(4): 467-73.
  • Martinez-Ruiz G, et al. (2008) Role of Smac/DIABLO in cancer progression. J Exp Clin Cancer Res. 27: 48.
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    货号: HG10339-CF
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