( We provide with SMPDL3A qPCR primers for gene expression analysis, HP102245 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 ASM3A / SMPDL3A 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG13559-ACG|
|人 ASM3A / SMPDL3A 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG13559-ACR|
|人 ASM3A / SMPDL3A 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG13559-CF|
|人 ASM3A / SMPDL3A 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG13559-CH|
|人 ASM3A / SMPDL3A 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG13559-CM|
|人 ASM3A / SMPDL3A 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG13559-CY|
|人 ASM3A / SMPDL3A 基因ORF全长cDNA(克隆载体)||HG13559-G|
|人 ASM3A / SMPDL3A 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG13559-NF|
|人 ASM3A / SMPDL3A 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG13559-NH|
|人 ASM3A / SMPDL3A 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG13559-NM|
|人 ASM3A / SMPDL3A 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG13559-NY|
|人 ASM3A / SMPDL3A 基因ORF全长cDNA克隆(表达载体)||HG13559-UT|
SMPDL3A gene is a novel liver X receptor (LXR) -regulated gene, with an LXR response element within its promoter. The induction of SMPDL3A is LXR-dependent and is restricted to human blood cells with no induction observed in mouse cellular systems. LXR α and LXRβ function as physiological sensors of cholesterol metabolites (oxysterols), regulating key genes involved in cholesterol and lipid metabolism. LXRs have been extensively studied in both human and rodent cell systems, revealing their potential therapeutic value in the contexts of atherosclerosis and inflammatory diseases. The LXR genome landscape has been investigated in murine macrophages but not in human THP-1 cells, which represent one of the frequently used monocyte/macrophage cell systems to study immune responses.