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人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签

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Human SMYD3 cDNA Clone产品信息
NCBI注册码:NM_001167740.1
参考序列ORF长度:1287bp
cDNA基因描述:Full length Clone DNA of Homo sapiens SET and MYND domain containing 3 transcript variant 1 with N terminal Flag tag.
分子别称:KMT3E, ZMYND1, ZNFN3A1, FLJ21080, MGC104324, bA74P14.1, SMYD3
分子种属:Human
载体:pCMV3-N-FLAG
质粒:
限制性酶切位点:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签 on other vectors
人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG11230-ACG1950
人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG11230-ACR1950
人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG11230-ANG1950
人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG11230-ANR1950
人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG11230-CF760
人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-His 标签HG11230-CH1770
人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG11230-CM1770
人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG11230-CY1770
人 SMYD3 transcript variant 1 基因ORF全长cDNA(克隆载体)HG11230-G760
人 SMYD3 transcript variant 1 基因ORF全长cDNA(克隆载体)HG11230-M760
人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG11230-NF1770
人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-His 标签HG11230-NH1770
人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG11230-NM1770
人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG11230-NY1770
人 SMYD3 transcript variant 1 基因ORF全长cDNA克隆(表达载体)HG11230-UT1770
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研究背景

SET and MYND domain-containing protein 3, also known as Zinc finger MYND domain-containing protein 1, SMYD3, and ZMYND, is a member of the histone-lysine methyltransferase family. SMYD3 contains one MYND-type zinc finger and one SET domain. SMYD3 is a histone H3 lysine-4-specific methyltransferase. It is expressed in skeletal muscles and testis. It is overexpressed in a majority of colorectal carcinoma (CRC) and hepatocellular carcinoma (HCC). SMYD3 plays an important role in transcriptional regulation in human carcinogenesis. It activates the transcription of a set of downstream genes. Of these downstream genes, there are several oncogenes and genes associated with cell adhesion (including those of N-Myc, CrkL, Wnt10b, L-selectin, CD31 and galectin-4), which have been shown to have effects on cell viability, adhesion, migration and metastasis. Increased SMYD3 expression is essential for the proliferation of breast cancer cells. SMYD3 may be a promising new target of therapeutic intervention for the treatment of cancers or other pathological processes associated with cell adhesion and migration.

参考资料
  • Hamamoto, R. et al., 2006, Cancer Sci. 97 (2): 113-8.
  • Luo, XG. et al., 2007, J Biosci Bioeng. 103 (5): 444-50.
  • Wang, XQ. et al., 2007, Exp Oncol. 29 (1): 71-3.
  • Silva, FP. et al., 2008, Oncogene. 27 (19): 2686-92.
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