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人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签

  • Human SPARCL1 / SPARC-like 1 Gene Expression validated Image 16680
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 SPARCL1 cDNA Clone产品信息
NCBI注册码:NM_004684.4
参考序列ORF长度:1995bp
cDNA基因描述:Full length Clone DNA of Homo sapiens SPARC-like 1 (hevin), transcript variant 2 with C terminal Flag tag.
分子别称:SC1, PIG33, SPARCL1
分子种属:Human
载体:pCMV3-C-FLAG
质粒:
限制性酶切位点:
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with SPARCL1 qPCR primers for gene expression analysis, HP100101 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签 on other vectors
人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG10046-ACG2070
人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG10046-ACR2070
人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10046-CF1890
人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-His 标签HG10046-CH1890
人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG10046-CM1890
人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG10046-CY1890
人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA(克隆载体)HG10046-M920
人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10046-M-F1890
人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG10046-NF1890
人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-His 标签HG10046-NH1890
人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG10046-NM1890
人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG10046-NY1890
人 SPARCL1 / SPARC-like 1 transcript variant 2 基因ORF全长cDNA克隆(表达载体)HG10046-UT1890
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研究背景

SPARC-like protein 1 (SPARCL1; also known as SC1, high endothelial venule protein, or hevin) is an extracellular matrix-associated, secreted glycoprotein belonging to the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins. It contains three conserved structural domains that are implicated in the regulation of cell adhesion, migration, and proliferation. SPARCL1 is expressed during embryogenesis and tissue remodeling and is especially prominent in brain and vasculature. Its down-regulation in a number of cancers and the possibility of its functional compensation by SPARC has led to recent interest in hevin as a tumor suppressor and regulator of angiogenesis. SPARCL1 has antiadhesive properties, and loss of SPARCL1 expression is associated with increased proliferative activity and cell cycle progression. It is suggested that it may influence multiple cellular processes during distinct stages of brain development and function. In addition, SPARCL1 can influence the function of astroglial cells in the developing and mature central nervous system (CNS).

参考资料
  • Sullivan MM, et al. (2004) Hevin/SC1, a matricellular glycoprotein and potential tumor-suppressor of the SPARC/BM-40/Osteonectin family. Int J Biochem Cell Biol. 36(6): 991-6.
  • Esposito I, et al. (2007) Tumor-suppressor function of SPARC-like protein 1/Hevin in pancreatic cancer. Neoplasia. 9(1): 8-17.
  • Weimer JM, et al. (2008) A BAC transgenic mouse model to analyze the function of astroglial SPARCL1 (SC1) in the central nervous system. Glia. 56(9): 935-41.
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