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人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-HA 标签

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Human SPG21 cDNA Clone产品信息
NCBI注册码:NM_016630.3
参考序列ORF长度:927bp
cDNA基因描述:Full length Clone DNA of Homo sapiens spastic paraplegia 21 (autosomal recessive, Mast syndrome), transcript variant 1 with N terminal HA tag.
分子别称:MAST, ACP33, GL010, BM-019, MASPARDIN
分子种属:Human
载体:pCMV3-N-HA
质粒:
限制性酶切位点:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列信息:
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-HA 标签 on other vectors
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签HG10522-ACG1950
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签HG10522-ACR1950
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签HG10522-ANG1950
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签HG10522-ANR1950
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10522-CF1770
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-His 标签HG10522-CH1770
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签HG10522-CM1770
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-HA 标签HG10522-CY1770
人 SPG21 transcript variant 1 基因ORF全长cDNA(克隆载体)HG10522-M760
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签HG10522-M-F1770
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签HG10522-NF1770
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-His 标签HG10522-NH1770
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签HG10522-NM1770
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体), N-HA 标签HG10522-NY1770
人 SPG21 transcript variant 1 基因ORF全长cDNA克隆(表达载体)HG10522-UT1770
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研究背景

Spastic paraplegia 21 (SPG21), also known as acid Cluster Protein 33 (ACP33) and Mast syndrome protein, is a member of the AB hydrolase superfamily. Human SPG21 is a 308 amino acid residue protein widely expressed in all tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. SPG21 binds to the hydrophobic C-terminal amino acids of CD4 which are involved in repression of T cell activation via the noncatalytic alpha/beta hydrolase fold domain. SPG21 thus is proposed to play a role as a negative regulatory factor in CD4-dependent T-cell activation of CD4. Defects in SPG21 are the cause of spastic paraplegia autosomal recessive type 21, also known as Mast syndrome, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. SPG21 is also associated with dementia and other central nervous system abnormalities.

参考资料
  • Zeitlmann L. et al., 2001, J Biol Chem. 276: 9123-32.
  • Simpson M. A. et al., 2003, Am J Hum Genet. 73: 1147-156.
  • Ota T. et al., 2004, Nat. Genet.36: 40-45.
  • Kedmi M. et al., 2007, Physiol Genomics. 28: 213-22.
  • Hanna M. C. et al., 2009, Neurogenetics.10: 217-28.
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    货号: HG10522-NY
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    库存2-3 weeks
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