( We provide with STK40 qPCR primers for gene expression analysis, HP101460 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 STK40 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG11597-ACG|
|人 STK40 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG11597-ACR|
|人 STK40 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG11597-ANG|
|人 STK40 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG11597-ANR|
|人 STK40 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG11597-CF|
|人 STK40 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG11597-CH|
|人 STK40 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG11597-CM|
|人 STK40 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG11597-CY|
|人 STK40 基因ORF全长cDNA(克隆载体)||HG11597-M|
|人 STK40 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG11597-NF|
|人 STK40 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG11597-NH|
|人 STK40 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG11597-NM|
|人 STK40 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG11597-NY|
|人 STK40 基因ORF全长cDNA克隆(表达载体)||HG11597-UT|
STK40 localized to both the cytoplasm and the nucleus. It is ubiquitously expressed. Mechanistically, Stk40 interacts with Rcn2, which also activates Erk1/2 to induce ExEn specification in mouse ESCs. Stk40 is able to activate the Erk/MAPK pathway and induce extraembryonic-endoderm (ExEn) differentiation in mouse ESCs. Interestingly, cells overexpressing Stk40 exclusively contribute to the ExEn layer of chimeric embryos when injected into host blastocysts. In contrast, deletion of Stk40 in ESCs markedly reduces ExEn differentiation in vitro. STK40 has a central serine/threonine protein kinase domain and is homologous to TRB-3, a protein that regulates activation of MAP kinases and inhibits NFκB-mediated gene transcription. Similarly, overexpression of STK40 inhibits NFκB activation triggered by TNF and also inhibits p53-mediated transcription. There are four named isoforms of STK40 that are produced as a result of alternative splicing.