|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 SUB1 / PC4 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG14326-ACG|
|人 SUB1 / PC4 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG14326-ACR|
|人 SUB1 / PC4 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG14326-ANG|
|人 SUB1 / PC4 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG14326-ANR|
|人 SUB1 / PC4 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG14326-CF|
|人 SUB1 / PC4 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG14326-CH|
|人 SUB1 / PC4 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG14326-CM|
|人 SUB1 / PC4 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG14326-CY|
|人 SUB1 / PC4 基因ORF全长cDNA(克隆载体)||HG14326-G|
|人 SUB1 / PC4 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG14326-NF|
|人 SUB1 / PC4 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG14326-NH|
|人 SUB1 / PC4 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG14326-NM|
|人 SUB1 / PC4 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG14326-NY|
|人 SUB1 / PC4 基因ORF全长cDNA克隆(表达载体)||HG14326-UT|
SUB1 belongs to the transcriptional coactivator PC4 family. It is a general coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. SUB1 binds single-stranded DNA. Single-stranded DNA-binding proteins play many roles in nucleic acid metabolism, but their importance during transcription remains unclear. SUB1 exhibits strong genetic interactions with factors necessary for promoter melting. It localizes near the transcription bubble in vitro and binds to promoters in vivo dependent upon preinitiation complexes assembly. SUB1 interacts with the nontemplate strand of RNApII complexes during initiation. It may also be involved in stabilizing the multiprotein transcription complex.