|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|人 SUMO1/SUMO-1 基因ORF全长cDNA克隆(表达载体), C-GFPSpark 标签||HG13095-ACG|
|人 SUMO1/SUMO-1 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签||HG13095-ACR|
|人 SUMO1/SUMO-1 基因ORF全长cDNA克隆(表达载体), N-GFPSpark 标签||HG13095-ANG|
|人 SUMO1/SUMO-1 基因ORF全长cDNA克隆(表达载体), N-OFPSpark 标签||HG13095-ANR|
|人 SUMO1/SUMO-1 基因ORF全长cDNA克隆(表达载体), C-Flag 标签||HG13095-CF|
|人 SUMO1/SUMO-1 基因ORF全长cDNA克隆(表达载体), C-His 标签||HG13095-CH|
|人 SUMO1/SUMO-1 基因ORF全长cDNA克隆(表达载体), C-Myc 标签||HG13095-CM|
|人 SUMO1/SUMO-1 基因ORF全长cDNA克隆(表达载体), C-HA 标签||HG13095-CY|
|人 SUMO1/SUMO-1 基因ORF全长cDNA(克隆载体)||HG13095-G|
|人 SUMO1/SUMO-1 基因ORF全长cDNA克隆(表达载体), N-Flag 标签||HG13095-NF|
|人 SUMO1/SUMO-1 基因ORF全长cDNA克隆(表达载体), N-His 标签||HG13095-NH|
|人 SUMO1/SUMO-1 基因ORF全长cDNA克隆(表达载体), N-Myc 标签||HG13095-NM|
|人 SUMO1/SUMO-1 基因ORF全长cDNA克隆(表达载体), N-HA 标签||HG13095-NY|
|人 SUMO1/SUMO-1 基因ORF全长cDNA克隆(表达载体)||HG13095-UT|
Small ubiquitin-like modifier protein (SUMO) modification is a highly dynamic process, catalyzed by SUMO-specific activating (E1), conjugating (E2) and ligating (E3) enzymes, and reversed by a family of SUMO-specific proteases (SENPs). Small ubiquitin-like modifier 1 (SUMO1) is a member of the superfamily of ubiquitin-like proteins. Despite its structural similarity with ubiquitin, SUMO1 does not seem to play any role in protein degradation. SUMO1 plays an important role in modulation of NOX activity required for ROS generation. SUMO1 haploinsufficiency results in cleft lip and palate in animal models. SUMO1 gene variation in human non-syndromic cleft lip with or without cleft palate (NSCLP) development. SUMO-1 may be useful as a novel target for therapy in oral squamous cell carcinoma (SCC) as well as a clinical indicator for tumor recurrence together with Mdm2.