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人 SerpinD1 基因ORF全长cDNA克隆(表达载体)

  • Human SerpinD1 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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 SERPIND1 cDNA Clone产品信息
NCBI注册码:NM_000185.3
参考序列ORF长度:1500bp
cDNA基因描述:Full length Clone DNA of Homo sapiens serpin peptidase inhibitor, clade D (heparin-cofactor), member 1.
分子别称:SerpinD1, HC2, LS2, HCF2, HCII, HLS2, D22S673
分子种属:Human
载体:pCMV3-untagged
质粒:pCMV3-SerpinD1
限制性酶切位点:KpnI + XbaI (6.1kb + 1.5kb)
Tag序列:
序列信息:Identical with the Gene Bank Ref. ID sequence except for a point mutation 1446 T/C not causing the amino acid variation.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with SerpinD1 qPCR primers for gene expression analysis, HP100340 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Ampicillin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
 SERPIND1 基因质粒图
Human SerpinD1 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
Product nameProduct name
研究背景

SerpinD1, also known as heparin cofactor II (HCâ…¡), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

参考资料
  • Rau JC, et al. (2009) Heparin cofactor II in atherosclerotic lesions from the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) study. Exp Mol Pathol. 87(3): 178-83.
  • Aihara K, et al. (2009) Heparin cofactor II as a novel vascular protective factor against atherosclerosis. J Atheroscler Thromb. 16(5): 523-31.
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    货号: HG10295-UT
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    Datasheet & Documentation

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