Syndecan-2 cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG10355-CF
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Syndecan-2 cDNA ORF Clone, Human, C-DDK (Flag®) tag 基本信息
基因
种属
Human
NCBI 参考序列号
参考序列ORF长度
606 bp
序列描述
Identical with the Gene Bank Ref. ID sequence.
描述
Full length Clone DNA of Human syndecan 2 with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pCMV3-C-FLAG
限制性酶切位点
KpnI + XbaI (6kb + 0.66kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输
Each tube contains lyophilized plasmid.
储存
The lyophilized plasmid can be stored at ambient temperature for three months.
Syndecan-2 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Syndecan-2 cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images
Human Syndecan-2/SDC2 Gene Expression validated Image 1506
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
other Green fluorescent protein / GFP Gene Plasmid Map 5609
Syndecan-2 cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
CD362 cDNA ORF Clone, Human;HSPG cDNA ORF Clone, Human;HSPG1 cDNA ORF Clone, Human;SYND2 cDNA ORF Clone, Human
Syndecan-2 Background Information
Full Name
syndecan 2

Syndecan-2 cDNA ORF Clone, Rat, Related Products

Product Name Catalog#(PDF)
Syndecan-2 cDNA ORF Clone in Cloning Vector, Rat RG83274-U
Syndecan-2 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG83274-ACGLN

Syndecan-2 cDNA ORF Clone, Mouse, Related Products

Product Name Catalog#(PDF)
Syndecan-2 cDNA ORF Clone in Cloning Vector, Mouse MG51047-G
Syndecan-2 cDNA ORF Clone, Mouse, untagged MG51047-UT
Syndecan-2 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51047-ACG
Syndecan-2 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51047-ACGLN
Syndecan-2 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51047-ACR
Syndecan-2 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51047-CF
Syndecan-2 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51047-NF
Syndecan-2 cDNA ORF Clone, Mouse, C-His tag MG51047-CH
Syndecan-2 cDNA ORF Clone, Mouse, N-His tag MG51047-NH
Syndecan-2 cDNA ORF Clone, Mouse, C-HA tag MG51047-CY
Syndecan-2 cDNA ORF Clone, Mouse, N-HA tag MG51047-NY
Syndecan-2 cDNA ORF Clone, Mouse, C-Myc tag MG51047-CM
Syndecan-2 cDNA ORF Clone, Mouse, N-Myc tag MG51047-NM
Syndecan-2 qPCR Primer Pairs, Mouse MP201005

Syndecan-2 cDNA ORF Clone, Human, Related Products

Product Name Catalog#(PDF)
Syndecan-2 cDNA ORF Clone in Cloning Vector, Human HG10355-M
Syndecan-2 cDNA ORF Clone, Human, untagged HG10355-UT
Syndecan-2 cDNA ORF Clone, Human, C-GFPSpark® tag HG10355-ACG
Syndecan-2 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10355-ACGLN
Syndecan-2 cDNA ORF Clone, Human, C-OFPSpark® tag HG10355-ACR
Syndecan-2 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10355-NF
Syndecan-2 cDNA ORF Clone, Human, C-His tag HG10355-CH
Syndecan-2 cDNA ORF Clone, Human, N-His tag HG10355-NH
Syndecan-2 cDNA ORF Clone, Human, C-HA tag HG10355-CY
Syndecan-2 cDNA ORF Clone, Human, N-HA tag HG10355-NY
Syndecan-2 cDNA ORF Clone, Human, C-Myc tag HG10355-CM
Syndecan-2 cDNA ORF Clone, Human, N-Myc tag HG10355-NM
Syndecan-2 qPCR Primer Pairs, Human HP100391
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